Research Article
POU2F1 Promotes Cell Viability and Tumor Growth in Gastric Cancer through Transcriptional Activation of lncRNA TTC3-AS1
Figure 7
The oncogenic function of POU2F1 in GC cells was mediated by lncRNA TTC3-AS1. SGC7901 cells were divided into three groups, NC, POU2F1-OE, and POU2F1-OE + TTC3-AS1-KD, and rescue experiments were performed. The RNA expression of (a) lncRNA TTC3-AS1 and (b) POU2F1 was detected by using qRT-PCR. (c) Cell viability was detected by using CCK-8 assays, and cell migration and invasion were, respectively, detected by using (d) scratch assay and (e, f) transwell assay. (g) Protein expression of POU2F1, E-cad, and FN1 was detected by using western blot. (f) The effects of POU2F1 knockdown or overexpression on lncRNA TTC3-AS1 expression was evaluated by using qRT-PCR. All experiments were performed in triplicate. The results were expressed as mean ± SD. , , and versus NC group., , and versus POU2F1-OE group.
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