E2F1 Maintains Gastric Cancer Stemness Properties by Regulating Stemness-Associated Genes

Figure 2

Enrichment of gastric cancer stem-like cells. (a) Tumor serum-free culture. (b) The mRNA expression level of stemness-associated genes in parental and tumor sphere cells. (c) Immunofluorescence staining of CD44 in parental adherent cells and tumor sphere cells. (d) The mRNA expression level of E2F1 in parental and tumor sphere cells. (e) The protein expression level of E2F1 in parental and tumor sphere cells.

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