Research Article
LncRNA PCNAP1 Promotes Hepatoma Cell Proliferation through Targeting miR-340-5p and is Associated with Patient Survival
Figure 4
PCNAP1 acts as a ceRNA to sponge miR-340-5p, which has been confirmed to inhibit ATF7 expression in HCC. (a) The binding sites of PCNAP1 and ATF7 to miR-340-5p, respectively, and the mutation of the binding sites. ((b) and (d)) Bars show the luciferase activity in HEK293 cells. Wild type denotes the wild-type binding site. Mutant denotes the mutant binding site. (c) Bars reveal the relative expression of miR-340-5p in SNU-182 cells, detected by qRT-PCR, and U6 served as the internal control. (e) Bars show the relative expression of ATF7 in SNU-182 cells, detected by qRT-PCR, and GAPDH served as the internal control. (f) Western blotting shows the protein expression of ATF7 in SNU-182 cells, and GAPDH served as the internal control. “”, ; “”, ; “”, .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |