MCT4 Promotes Tumor Malignancy in F98 Glioma Cells

Figure 1

Endogenous MCT4 expression in GBM and F98 glioma cells. (a) Relative MCT4 mRNA expression in GBM normalized to con as determined by qRT-PCR. Statistical analysis was performed by Student’s t-test (, mean ± SEM, n = 7). (b) MCT4 expression in different glioma subtypes. Data were obtained from the GENT2 database. Statistical analysis was performed by Student’s t-test with FDR correction for multiple testing (, mean ± SEM, n > 26). (c) Survival curves of patients with different MCT4 expression levels in GBM. High and low expressions are defined as above and below the median expression, respectively. Data were obtained from the GENT2 database. Statistical analysis was performed by log-rank test (, mean ± SEM, n = 25). (d) Scheme of MCT4 regulation by hypoxia via HIF-1α and gene expression analysis of MCT4/SLC16A3 and HIF-1α/HIF1A in histological GBM compartments, shown as normalized gene-level FPKM values (n = 278). Data were obtained from the IVY Glioblastoma Atlas Project database. (e) Network analysis for highest interactors with MCT4/SLC16A3. Shown are all connections between interactors. Data were obtained from the STRING database. (f) Relative MCT4 mRNA expression in F98 wild-type (wt) cells cultured under hypoxic conditions and normalized to normoxic cell culture conditions as determined by qRT-PCR. Statistical analysis was performed with Student’s t-test (, mean ± SEM, n = 3).

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