MCT4 Promotes Tumor Malignancy in F98 Glioma Cells

Figure 6

Cell cycle profiles and cell death mechanisms in MCT4 overexpression and knockdown F98 glioma cells. (a) Cell cycle analysis of con, MCT4, and MCT4^KD F98 cells, as determined by flow cytometry analysis. Statistical analysis was performed by two-way ANOVA with Bonferroni posttest (, , and , mean ± SEM, n = 3). (b) Cell death mechanisms in con, MCT4, and MCT4^KD F98 cells, as determined by flow cytometry analysis. Viable cells are defined as both PI- and Annexin V-negative, damaged cells are defined as PI-positive and Annexin V-negative, apoptotic cells are defined as PI-negative and Annexin V-positive, and late apoptotic/necrotic cells are defined as both PI- and Annexin V-positive. Statistical analysis was performed by two-way ANOVA with Bonferroni posttest (; , mean ± SEM, n = 3). (c) Ferroptosis in con, MCT4, and MCT4^KD F98 cells treated with 10 μM erastin and 1 μM ferrostatin, as determined by MTT assay. Data were normalized to untreated controls. Statistical analysis was performed by one-way ANOVA with Bonferroni posttest (^ns; , mean ± SEM, n = 3).

(a)

(b)

(c)