Id4 Suppresses the Growth and Invasion of Colorectal Cancer HCT116 Cells through CK18-Related Inhibition of AKT and EMT Signaling

Figure 4

Id4 binding to CK18 regulated the PI3K pathway and the expression of EMT-related markers. (a) Coomassie blue-stained SDS-PAGE gel showed the protein binding to Id4 (flag). (b) Co-IP assay showed the interaction between Id4 and CK18 in the HCT116 cells. (c) Id4 decreased CK18 protein expression (Western blotting). (d) HCT116-Id4 cells and negative control cells were transfected with CK18 plasmid or control plasmid, respectively. Western blotting was performed to investigate the effect of CK18 on the expression of EMT-related markers in the HCT116 cells.

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