Hsp70 Promotes SUMO of HIF-1α and Promotes Lung Cancer Invasion and Metastasis

Figure 5

Thermal stimulation upregulates the expression of Hsp70 and promotes lung cancer proliferation and metastasis. (a) Transwell detects the influence of thermal stimulation on the invasion ability of NCI-H446 (magnification 200x). (b) Transwell detects the effect of thermal stimulation on the invasion ability of A549 (magnification 200x). (c) qRT-PCR detects the effect of thermal stimulation on the expression of E-cadherin. (d) qRT-PCR detects the effect of thermal stimulation on vimentin expression. (e) Western blot to detect the effect of thermal stimulation on the expression of Hsp70. (f) Western blot to detect the effect of thermal stimulation on the expression of SUMO1 on HIF-1α. (g) Western blot detects the effect of thermal stimulation on the SUMO2/3 of HIF-1α. .

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