circITGA7 Acts as a miR-370-3p Sponge to Suppress the Proliferation of Prostate Cancer

Figure 1

Relative circITGA7 expression levels in PCa. (a) Clustered heat map of the differentially expressed circRNAs in three pairs of human PCa tissues and adjacent normal tissues (GSE179321). (b) Schematic illustration shows the circularization of ITGA7 exon 4 formed circITGA7. The back-splice junction of circITGA7 was identified by Sanger sequencing. (c) PCR assay with divergent or convergent primers indicated the existence of circITGA7 in PCa cells. Divergent primers ◀▶; convergent primers ▶◀. (d) The expression of circITGA7 and ITGA7 mRNA in PCa cells treated with RNase R was detected by qRT-PCR. (e) The expression of circITGA7 in 28 pairs of PCa tissues and paracancerous tissues was detected. (f) The relative expression of circITGA7 was detected using qRT-PCR in RWPE-1, PC3, LNCaP, and DU145 cells. (g) RNA-FISH indicated the location of circITGA7 in PC3 cells. Scale bar, 10 μm. Data were presented as mean ± SD of three independent experiments. Statistically significant differences were indicated: .

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