The lncRNA NEAT1/miRNA-766-5p/E2F3 Regulatory Axis Promotes Prostate Cancer Progression

Figure 7

NEAT1 regulated PCa progression by the miRNA-766-5p/E2F3 axis. We constructed stable cell lines via transfecting with sh-NEAT1, followed stimulated with miRNA-766-5p inhibitor and sh-E2F3. (a) The levels of miR-755-5p and E2F3 were detected by qRT-PCR. , miR-766-5p inhibitor vs. NC inhibitor, sh-E2F3 vs. sh-NC. (b) Cell viability was determined by CCK-8 analysis. , miR-766-5p inhibitor vs. NC inhibitor; , miR-766-5p inhibitor + sh-E2F3 vs. miR-766-5p inhibitor. (c) Cell proliferation was measured by colony formation analysis. , miR-766-5p inhibitor vs. NC inhibitor; , miR-766-5p inhibitor + sh-E2F3 vs. miR-766-5p inhibitor. (d) Cell apoptosis was evaluated by flow cytometry analysis. , miR-766-5p inhibitor vs. NC inhibitor; , miR-766-5p inhibitor + sh-E2F3 vs. miR-766-5p inhibitor. (e) Cell migration and invasion abilities were measured by the transwell assay (100×). , miR-766-5p inhibitor vs. NC inhibitor; , miR-766-5p inhibitor + sh-E2F3 vs. miR-766-5p inhibitor.

(a)

(b)

(c)

(d)

(e)