Epidermal Growth Factor Receptor as Target for Perioperative Elimination of Circulating Colorectal Cancer Cells

Figure 2

Binding of anti-EGFR mAbs to tumor cell lines and subsequent ADCP by macrophages. The anti-EGFR mAbs cetuximab (black bars), zalutumumab (light grey bars), and panitumumab (dark grey bars) were used for opsonization (a) of (A) A431, (B) Caco2, (C) HCT116, (D) HT29, (E) SW948, and (F) RKO cells. Concentrations were 30–10–5–2.5–1–0.5–0.1–0.05–0.01–0.005–0.001 μg/ml. MFI = mean fluorescence intensity. Phagocytosis (b) of (A) A431, (B) Caco2, (C) HCT116, (D) HT29, (E) SW948, and (F) RKO cells by human monocyte-derived macrophages in the presence of 0.1 μg/ml cetuximab (black bars), zalutumumab (light grey bars), and panitumumab (dark grey bars). Tumor cell killing was analyzed by flow cytometry. The percentage killing shown is relative to the no antibody control. Bars represent mean ± SEM. , .

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