Hsa_circ_0000994 Inhibits Pancreatic Cancer Progression by Clearing Immune-Related miR-27a and miR-27b

Figure 2

Silencing Hsa_circ_0000994 promoted carcinogenic properties of PANC1 cells. (a) RT-qPCR detection of the expression level of Hsa_circ_0000994 in PANC1 after transfection. (b) Detection of cell viability of transfected PANC1 by the CCK-8 method. (c) The clonogenic ability of PANC1 after transfection detected by clonogenic assay. (d) Apoptosis of PANC1 detected by flow cytometry. (e) Caspase-3/-9 activity in PANC1 after transfection. (f) The migration and invasion ability of transfected PANC1 cells detected by the transwell method. (g) Nude mice tumors after transfection of PANC1. (h) 15 days after injection, tumors were excised and weights were measured. (i) IHC detection of Ki67 expression and cell number. IHC, immunohistochemical. , , , , and , . Overexpression of Hsa_circ_0000994 inhibits cell proliferation, migration, and invasion.

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