Hsa_circ_0000994 Inhibits Pancreatic Cancer Progression by Clearing Immune-Related miR-27a and miR-27b

Figure 4

Hsa_circ_0000994 sponges miR-27a/-27b. (a) Schematic diagram of miR-27a/-27b binding sites in Hsa_circ_0000994. (b) Subcellular localization of Hsa_circ_0000994 and miR-27a/-27b detected by fluorescence in situ hybridization. (c) The expression of miR-27a/-27b in transfected PANC1 and SW1990 detected by RT-qPCR.(d) RT-qPCR detection of miR-27a/-27b expression in PC and normal tissue.(e) Luciferase reporter gene suggested that overexpression of miR-27a/-27b inhibited the activity of Hsa_circ_0000994 in PANC1 and SW1990. , and , . The tumor suppressor function of Hsa_circ_0000994 depends on miR-27a and miR-27b.

(a)

(b)

(c)

(d)

(e)