Neferine Exerts Ferroptosis-Inducing Effect and Antitumor Effect on Thyroid Cancer through Nrf2/HO-1/NQO1 Inhibition

Figure 3

Neferine induced the activation of ferroptosis in thyroid cancer cells. (a) The relative iron level was measured by an iron assay kit. and vs. control. (b) The ROS level was detected by flow cytometry after IHH-4 and CAL-62 cells were treated with DCHF-DA. and vs. control. (c) The relative protein expressions of both SLC7A11 and GPX4 were determined by the western blot. The data were expressed after being normalized to β-actin. and vs. control. (d) The levels of GSH were quantified by using the commercial kit. and vs. control. (e–g) After IHH-4 and CAL-62 cells were stimulated with Fer-1, neferine or their combination, the level of Fe²⁺, ROS production, and the relative level of GSH were assessed by using the iron assay kit, flow cytometry, and commercial kit, respectively. and . All assays were performed in triplicate.

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