Neferine Exerts Ferroptosis-Inducing Effect and Antitumor Effect on Thyroid Cancer through Nrf2/HO-1/NQO1 Inhibition

Figure 6

Neferine impeded tumorigenesis and induced ferroptosis in vivo involving in the Nrf2/HO-1/NQO1 pathway. (a) Representative images of tumors from nude mice in the control group and neferine 20 mg/kg group. (b, c) The tumor volumes and mice body weight were monitored every five days. . (d) Tumor samples were weighted after mice were administrated with 30 days. vs. 0 mg/kg neferine. (e–g) The apoptosis and VEGF expression were detected by TUNEL and IHC, respectively. vs. 0 mg/kg neferine. (h) The relative protein expressions of SLC7A11 and GPX4 were examined by the western blot. The data were expressed after being normalized to β-actin. vs. control. (i) The relative protein expressions of Nrf2, HO-1, and NQO1 were assessed by the western blot. The data were expressed after being normalized to lamin B1 or β-actin. and vs. control. (j, k) The levels of ROS (j) and GSH (k) were measured using commercial kits. and vs. 0 mg/kg neferine. All assays were performed in triplicate.

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