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Journal of Obesity
Volume 2010, Article ID 371950, 10 pages
Research Article

Feeding Regulates the Expression of Pancreatic Genes in Gastric Mucosa

Functional Genomics Laboratory, CREMOGH, CRCHUQ, and Department of Molecular Medicine, Laval University, 2705 Boulevard Laurier, Quebec city, QC, Canada G1V 4G2

Received 5 September 2010; Revised 19 November 2010; Accepted 23 November 2010

Academic Editor: Xu Feng Huang

Copyright © 2010 Maria Rita De Giorgio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The ineffective short-term control of feeding behavior compromises energy homeostasis and can lead to obesity. The gastrointestinal tract secretes several regulatory peptides. However, little is known about the stomach peptide contribution to the acute regulation of intake. In an attempt to identify new gastric signals, the serial analysis of gene expression (SAGE) method was used for the transcription profiling of stomach mucosa in 7 groups of mice: fasting and sacrificed 30 minutes, 1 hour, 3 hours after a low-fat (LF) or high-fat (HF) ad libitum meal. In total, 35 genes were differentially modulated by LF and HF meals compared to fasting, including 15 mRNAs coding for digestive enzymes/secretory proteins, and 10 novel transcripts. Although the basic expression profile did not undergo substantial variations, both LF and HF meals influenced the transcription. This study represents the first global analysis of stomach transcriptome as induced by different nutritional stimuli. Further studies including the characterization of novel genes may help to identify new targets for the therapy and prevention of obesity.