A Model of Insulin Resistance in Mice, Born to Diabetic Pregnancy, Is Associated with Alterations of Transcription-Related Genes in Pancreas and Epididymal Adipose Tissue

Table 1

Gene regions amplified and their corresponding primer sequences used for RT-PCR.

Genes amplified	Primer sequences
Mouse RANTES	F: 5^′-GCAGTCGTGTTTGTCACTCG-3^′
	R: 5^′-TAGGACTAGAGCAAGCGATGAC-3^′
Mouse CCR5	F: 5^′-GCCTAAACCCTGTCATCTATGC-3^′
	R: 5^′-ATATTTCCCGGCCCTGATAAAAG-3^′
Mouse MCP-1	F: 5^′-GAGAGCCAGACGGGAGGAAG-3^′
	R: 5^′-TGAATGAGTAGCAGCAGGTGAG-3^′
Mouse CD68	F: 5^′-TTCAGGGTGGAAGAAAGGTAAAGC-3^′
	R: 5^′-CAATGATGAGAGGCAGCAAGAGG-3^′
Mouse IL-6	F: 5^′-CCGCTATGAAGTTCCTCTCTGC-3^′
	R: 5^′-ATCCTCTGTGAAGTCTCCTCTCC-3^′
Mouse TCRα	F: 5^′-CCTCTACAGCAGCGTTCTCATCC-3^′
	R: 5^′-GGGTAGGTGGCGTTGGTCTCTTTG-3^′
Mouse CD14	F: 5^′-GCGTGTGCTTGGCTTGTTG-3^′
	R: 5^′-CAGGGCTCCGAATAGAATCCG-3^′
Mouse F4/80	F: 5^′-TCCAGCACATCCAGCCAAAGC-3^′
	R: 5^′-CCTCCACTAGCATCCAGAAGAAGC-3^′
Mouse TLR-2	F: 5^′-CTACAGTGAGCAGGATTCC-3^′
	R: 5^′-CAGCAAAACAAGGATGGC-3^′
Mouse TNF-α	F: 5^′-CTCTTCTCATTCCTGCTTGTGG-3^′
	R: 5^′-AATCGGCTGACGGTGTGG-3^′
Mouse SREBP-1c	F: 5^′-CATCAACAACCAAGACAGTC-3^′
	R: 5^′-CCAGAGAAGCAGAAGAGAAG-3^′
Mouse FAT/CD36	F: 5^′-TGCTCTCCCTTGATTCTGCTGC-3^′
	R: 5^′-TTTGCTGCTGTTCTTTGCCACG-3^′
Mouse Adiponectin	F: 5^′-GCCGCTTATGTGTATCGCTCAG-3^′
	R: 5^′-GCCAGTGCTGCCGTCATAATG-3^′
Mouse Leptin	F: 5^′-ACACACGCAGTCGGTATCC-3^′
	R: 5^′-GAGTAGAGTGAGGCTTCCAGG-3^′
Mouse Glucokinase	F: 5^′-AGAAGGCTCAGAAGTTGGAGAC-3^′
	R: 5^′-GGATGGAATACATCTGGTGTTTCG-3^′
Mouse Insulin	F: 5- TGGCTTCTTCTACACACCCAT-3^′
	R: 5^′-CTCCAGTGCCAAGGTCTGAA-3^′
Mouse Glut2	F: 5^′-TGTGGTGTCGCTGTTTGTTG-3^′
	R: 5^′-AATGAAGTTTGAGGTCCAGTTGG-3^′
Mouse C/EPB-β	F: 5^′-AGCTGAGCGACGAGTACAAG-3^′
	R: 5^′-AGCTGCTCCACCTTCTTCTG-3^′
Mouse Maf-A	F: 5^′-ATCACTCTGCCCACCATCAC-3^′
	R: 5^′-CGCCAACTTCTCGTATTTCTCC-3^′
Mouse Nkx6-1	F: 5^′-GGGTCTTCCTCCTCCTCCTC-3^′
	R: 5^′-GGTCTGGTGTGTTTTCTCTTCC-3^′
Mouse Pdx-1	F: 5^′-CTACTGCCTTCGGGCCTTAG-3^′
	R: 5^′-TTGGAACGCTCAAGTTTGTACC-3^′