Catechins and Caffeine Inhibit Fat Accumulation in Mice through the Improvement of Hepatic Lipid Metabolism
Figure 4
Effects of catechins, EGCG, and caffeine on the mRNA expression of FAS, ACO, and CPT II in liver in mice. (a1) Fluorescence intensity of mRNA of FAS, ACO, and CPT II by RT-PCR analysis in liver in mice fed the diets containing 0.3% catechins, 0.05% caffeine, 0.3% catechins + 0.05% caffeine for 4 weeks. Lane 1: control, lane 2: 0.3% catechins, lane 3: 0.05% caffeine, lane 4: 0.3% catechins + 0.05% caffeine. (b1) Fluorescence intensity of mRNA of FAS, ACO, and CPT II by RT-PCR analysis in liver in mice fed the diets containing 0.1% EGCG, 0.05% caffeine, 0.1% EGCG + 0.05% caffeine for 4 weeks. Lane 1: control, lane 2: 0.1% EGCG, lane 3: 0.05% caffeine, lane 4: 0.1% EGCG + 0.05% caffeine. (a2 and b2) Expression levels of mRNA of FAS, ACO, and CPT II determined by RT-PCR analysis in liver in mice fed the diets containing catechins and caffeine (a2) and EGCG and caffeine (b2) for 4 weeks. The level was determined from the fluorescence intensity by a image analysis system and showed as a relative value to the control. Results are given as mean ± SE of 8 mice in each groups. *Significant difference compared with the control ().
