Figure 2: Dose response of hemodynamic measurements of acute nisoxetine or saline. Animals underwent a 24 h food deprivation prior to injections and refeeding. All animals were fed and maintained on standard chow (13% fat, 27% protein). Doses were randomly administered with a week washout between doses and tail-cuff volume pressure recording were performed at 1 h, 4 h, and 24 h. (a). Mean blood pressure (b). Systolic blood pressure (c). Diastolic blood pressure (d). Heart rate. Asterisks indicate difference from saline ( ) at the individual time points.