Figure 4: Diet-induced changes to the feeding suppression of a subthreshold nisoxetine dose. Following a 10-week feeding of high-fat or control diets, all rats were given a 24 h food deprivation and then fed standard chow. Animals were injected (IP) with either nisoxetine (3 mg/kg) or saline. One week later the injections were counterbalanced. Rats exposed to a high-fat diet showed a higher sensitivity to feeding suppression of nisoxetine at 4 and 24 hours. Animals exposed to the high-fat diet had an overall reduced intake of chow at 24 h, which is indicated by * ( ). Similar letters indicate significance from each other ( ). # indicates difference ( ) from all groups at the individual time points.