Figure 5: Representative coronal micrographs of immunohistochemistry c-Fos staining (black) in the arcuate nucleus of the hypothalamus. Animals exposed to a high-fat diet were injected with either nisoxetine (3 mg/kg; (a)) or saline (b) following a 24 h food deprivation. Animals were sacrificed 2 h after injection. The bar in each image represents 160 μm. Coordinates for the representative sections were 3 mm caudal from bregma. Abbreviation: V3 = third ventricle.