Methylation and Expression of Immune and Inflammatory Genes in the Offspring of Bariatric Bypass Surgery Patients

Table 2

Comparison of the top 25 overrepresented canonical immune/inflammatory pathways identified from differential methylation and expression analyses between BMS and AMS siblings.

IPA canonical pathways	P value	𝑁 of diff. meth. genes	P-value	𝑁 of Diff. Express. genes
CD28 signaling in T-helper cells	4.24×10^−7	54	0.40	5
Leukocyte extravasation signaling	6.34×10^−7	78	0.25	13
Role of NFAT in cardiac hypertrophy	6.98×10^−7	76	NI	—
T-Cell receptor signaling	7.55×10^−7	47	0.07	7
Dendritic cell maturation	8.22×10^−7	66	0.10	9
Clathrin-mediated endocytosis signaling	1.24×10^−6	74	0.09	11
IL-8 signaling	𝟐.𝟒𝟒×𝟏𝟎^−𝟔	70	0.03	12
iCOS-iCOSL signaling in T-helper cells	𝟓.𝟑𝟓×𝟏𝟎^−𝟔	48	0.01	9
Virus entry via endocytic pathways	5.48×10^−6	43	NI	—
NF-κB signaling	6.08×10^−6	66	0.08	10
Role of NFAT in regulation of the immune response	𝟖.𝟏𝟒×𝟏𝟎^−𝟔	69	0.02	12
PKCθ signaling in T lymphocytes	9.18×10^−6	50	0.24	6
Macropinocytosis signaling	2.5×10^−5	32	NI	—
CCR5 signaling in macrophages	2.66×10^−5	34	0.42	3
CTLA4 Signaling in Cytotoxic T Lymphocytes	2.71×10^−5	40	NI	—
B-Cell receptor signaling	𝟐.𝟗𝟔×𝟏𝟎^−𝟓	58	0.008	12
fMLP signaling in neutrophils	3.94×10^−5	47	0.05	8
RAR activation	4.32×10^−5	66	0.32	8
IL-1 signaling	6.55×10^−5	40	0.27	5
Glucocorticoid receptor signaling	𝟔.𝟕𝟐×𝟏𝟎^−𝟓	92	0.005	19

Pathways in bold are overrepresented in both gene methylation and expression data. Abbreviations: diff. express.: differentially expressed; diff. Meth.: differentially methylated; 𝑁: number; NI: not identified.