Association of Obesity with Proteasomal Gene Polymorphisms in Children

Table 1

General information about the genotyped loci.

Gene/chromosome location	Polymorphism			Genotyping method	Primers	Fragment size (bp)
	Variation	ID	Function
PSMA6/14q13.2	c.-110C>A	Rs2277460	Promoter	ASA	F: 5′-ATGCAAGAGCGGAAGAAAC-3′	256
					F: 5′-ATGCAAGAGCGGAAGAAAA-3′
					R: 5′-CTGAATTGCCCTGTCATGGTA-3′
	c.-8C>G	Rs1048990	5′UTR	CAPS/RsaI	F: 5′-ACGTTGGATGCTCCAGATGAAAGCCTGA-3′	256/161 + 94
					R: 5′-ACGTTGGATGGCCCTATCTTCCTTAACTCTC-3′
PSMA3/14q23	c. 543+138G>A	Rs2348071	Int7	CAPS/TscAI	F: 5′-GTCTAAGGCAGGGATGTCCA-3′	232/166 + 66
					R: 5′-ACCAGCTTTCCCATTCAGTG-3′

SNP: single nucleotide polymorphism.
ID: indicates GenBank polymorphic loci accession number.
ASA: allele specific amplification.
CAPS: cleaved amplified polymorphic site.
Bp: base pairs.
F: forward.
R: reverse.