Alterations in Phosphorylated CREB Expression in Different Brain Regions following Short- and Long-Term Morphine Exposure: Relationship to Food Intake

Figure 4

Changes in P-CREB staining in the VTA following different morphine treatments ((a) group 1, naïve; (b) group 2, saline; (c) group 3, MorX1; (d) group 5, MorX8; (e) group 6, Esc; (f) group 7, PP24 h; (g) group 8, PM24 h; (h) group 9, PP7d; (i) group 10 PM7d) as determined by immunofluorescence (200X). Quantification of P-CREB (j). See Table 1 for details on the morphine treatment paradigm. 𝑁=6--9 per treatment. *𝑃<0.005 versus saline injection, **𝑃<0.0001 versus saline injection, ^∧𝑃<0.05 versus 7 d placebo pellet.

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