Figure 2: Protein levels of HIF-1α (a) and phospho-AMPKα (b) in rat RpWAT during cold acclimation. Protein content is expressed relative to a room temperature-acclimated control, which was standardized as 100%. The results of the representative example from three observations are shown. Data were quantified as described in Section 2. The values represent the mean ± S.E.M. *Compared to control, , , and .