The Intrauterine and Nursing Period Is a Window of Susceptibility for Development of Obesity and Intestinal Tumorigenesis by a High Fat Diet in Min/+ Mice as Adults

Figure 10

The net effect on tumor size of (a) a 45% fat diet, and (b) of PhIP exposure. (a) The size distribution of small intestinal tumors from untreated pooled female and male Min/+ mice exposed to a 45% fat diet for various periods is calculated by subtracting the mean number of tumors in the mice exposed to a 10% fat diet throughout life from the mean number of tumors in the treatment groups receiving a 45% fat diet for various periods for each tumor size class. (b) The size distribution of small intestinal tumors from pooled female and male Min/+ mice exposed to PhIP is calculated by subtracting the mean number of spontaneous tumors in the mice not exposed to PhIP from the mean number of tumors formed in PhIP-treated mice for each tumor size class. The intervals between the tumor size classes are 0.25 mm. The symbols for the experimental groups are as follows: in (a) 10+10+10 (○), 45+10+10 (◊), 10+45+10 (□), 45+45+10 (●), 10+10+45 (◆) 45+45+45 (■), and in (b) the dietary groups without PhIP exposure; 45+45+10 (○), 45+45+45 (□), and the same PhIP-exposed dietary groups with (●) or (■), respectively. The experimental groups are as explained in the legend to Figure 1. .

(a)

(b)