Table of Contents Author Guidelines Submit a Manuscript
Journal of Obesity
Volume 2017 (2017), Article ID 6754734, 6 pages
https://doi.org/10.1155/2017/6754734
Research Article

Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

1Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy
2CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, Italy
3Dipartimento di Medicina e Chirurgia, Università di Salerno, Via Giovanni Paolo II 132, Fisciano, 84084 Salerno, Italy
4IRCCS SDN-Istituto di Ricerca Diagnostica e Nucleare, Via Gianturco 113, 80100 Naples, Italy
5Dipartimento di Scienze Biomediche Avanzate, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy
6Dipartimento di Medicina Clinica e Chirurgia, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy
7Dipartimento Scienze Motorie e del Benessere, Università di Napoli Parthenope, Via Amm. F. Acton 38, 80133 Naples, Italy

Correspondence should be addressed to Lucia Sacchetti

Received 28 December 2016; Revised 17 February 2017; Accepted 23 February 2017; Published 13 March 2017

Academic Editor: R. Prager

Copyright © 2017 Carmela Nardelli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women. Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation. Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.