Inhibition of Methylglyoxal-Mediated Protein Modification in Glyoxalase I Overexpressing Mouse Lenses
Glo I expression and activity in transgenic mouse lenses. (a) H&E staining of Wt and Glo I transgenic lenses showed no morphological changes in 6-month old animals. No difference was noticeable between the Wt and transgenic lens. (b) Immunohistochemistry using a monoclonal antibody for human Glo I showed human Glo I expression in epithelial cells and outer cortical fiber cells. Glo I immunoreactivity was absent in Wt lenses. (c) In situ hybridization shows Glo I mRNA in outer cortical and epithelial cells in Glo I transgenic animals. (d) Glo I activity was measured in the water-soluble lens proteins. Glo I-catalyzed formation of S-D-lactoylglutathione from MGO and GSH was monitored at 240 nm. The data are the mean SD from 4 lenses.
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