NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy

Figure 3

Apocynin reverses continuous high glucose-induced ROS production in BRP. Subconfluent cells in 24-well plate were exposed to normal glucose (NG, 5.6 mM) and continuous high glucose (HG, 25 mM) in the absence and presence of 500 $\mu$M apocynin (HGA) or 1 $\mu$M MitoQ (HGMQ) for 3 days. Approximately, 2 h before the end of incubation, DHE (10 $\mu$M) was added. After the treatment, the cells were washed twice with PBS, lysed and fluorescence (FL) intensity was measured. Data are presented as mean $\pm$ SEM of 6 separate experiments. ${}_{}{}^{*}P<.05$ versus NG, ${}_{}{}^{**}P<.05$ versus HG.