Figure 3: Apocynin reverses continuous high glucose-induced ROS production in BRP. Subconfluent cells in 24-well plate were exposed to normal glucose (NG, 5.6 mM) and continuous high glucose (HG, 25 mM) in the absence and presence of 500 μM apocynin (HGA) or 1 μM MitoQ (HGMQ) for 3 days. Approximately, 2 h before the end of incubation, DHE (10 μM) was added. After the treatment, the cells were washed twice with PBS, lysed and fluorescence (FL) intensity was measured. Data are presented as mean ± SEM of 6 separate experiments. P*<.05 versus NG, P**<.05 versus HG.