Figure 6: Apocynin reverses glucose-induced CML formation in BRP. Subconfluent cells in 24-well plate were exposed to normal glucose (NG, 5.6 mM) and continuous high glucose (HG, 25 mM) and in the presence of 500 μM apocynin (HGA) or 1 μM MitoQ (HGMQ) for 4 days. After incubation, the cells were washed twice with ice-cold PBS, lysed and CML were detected using competitive ELISA. Data are presented as Mean ± SEM of 8–13 samples. P*<.05 versus NG, P**<.05 versus HG.