Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct

Figure 8

Expression of NPC and lineage markers before and after transduction and differentiation. The effects of passage number, induction of differentiation and GDNF transgene expression on the expression of 5 markers was evaluated using ICC. Nontransduced and lenti-GDNF-GFP vector transduced cNPCp20 were cultured in UM or UM-FBS, then immunolabeled with specific antibodies. The changes in expression patterns seen predominantly reflected exposure to differentiation conditions (alternating columns), with little that might be attributable to passage number or lenti-GDNF-GFP transduction. Scale bar = 50 𝜇m.