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Journal of Ophthalmology
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Journal of Ophthalmology
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2011
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Article
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Tab 2
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Research Article
Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct
Table 2
Primary antibodies used for immunocytochemistry on cNPCs.
Target
Antibody type
Reactivity in retina
Source
Dilution
Nestin
Mouse monoclonal
Progenitors, reactive glia
BD
1 : 200
Vimentin
Mouse monoclonal
Progenitors, reactive glia
Sigma
1 : 200
Ki-67
Mouse monoclonal
Proliferating cells
BD
1 : 200
GFAP
Mouse monoclonal
Astrocytes, reactive glia
Chemicon
1 : 200
3-tubulin
Mouse monoclonal
Immature neurons
Chemicon
1 : 200
GDNF
Rabbit polyclonal
Growth factor
SCBT
1 : 200