Variables and Strategies in Development of Therapeutic Post-Transcriptional Gene Silencing Agents
Figure 9
Crystal structure analysis of allelic variant constructs. We analyzed the location of the F293C mutation in the bovine rod opsin crystal structure (1F88.pdb). WT protein (a). The image shows the cutout region around the protonated Schiff base linkage of 11-cis-retinal (orange) to K296 (blue sidechain). F293 is lavender in color. The disulfide bond between C110 and C187 is in yellow. F293 is within 5 Å of the Schiff base and within 10 Å of the disulfide bond. F293C Mutation (b). The C293 sidechain is lavender in color with a yellow tip indicating a free sulfhydryl group (-SH). The SH group is within 5 Å of the Schiff base and 10 Å of the disulfide bond.
