Potential Therapeutic Roles for Inhibition of the PI3K/Akt/mTOR Pathway in the Pathophysiology of Diabetic Retinopathy
Figure 2
Oxygen-induced retinopathy model-retinal flat mount. Seven-day old SV129 mouse pups were put at 70% oxygen for 5 days, removed to normal air, and injected intraperitoneal with Palomid 529 for 5 days. The animals were then sacrificed and the eyes removed and fixed in 10% formalin for 30 min. The retinas were isolated, blocked for 1 hr in PBS containing 1% BSA, 1% goat serum, and 0.5% TX-100, stained overnight with 10 μg/mL BS-1 lectin labeled with FITC (Sigma-Aldrich), washed in PBS and flat mounted.
