Conditional Gene Targeting: Dissecting the Cellular Mechanisms of Retinal Degenerations

Figure 1

Schematic diagram of generating a conditional knockout (CKO) mouse from breeding a tissue-specific Cre mouse (top right) with a mouse carrying homozygous floxed gene (top left). A CKO mouse carrying a homozygous floxed gene and cre (either heterozygous or homozygous) is obtained by genotyping the F2 offspring. Tissue-specific Cre expression is shown as grey-eared (top right). Tissue-specific gene KO is diagramed as black-eared (bottom).