Review Article

Multiphoton Microscopy for Ophthalmic Imaging

Figure 7

Second harmonic generation (SHG) and two-photon autofluorescence (2PAF) of TM region of a human eye from a 73-year-old donor. A section of the eye was flat-mounted with the anterior chamber facing the microscope objective. Images represent a projection of the multiple -sections flattened into a single plane. (a) The SHG emission (388 nm to 409 nm) collected from 800 nm excitation of TM. (b) The 2PAF emission window (452 nm to 644 nm) collected simultaneously. (c) Merged image of SHG (blue) and AF (green) emission. Black scale bar = 50 μm. This figure is reprinted from [36].
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