Review Article | Open Access
Ocular Surface Development and Gene Expression
The ocular surface—a continuous epithelial surface with regional specializations including the surface and glandular epithelia of the cornea, conjunctiva, and lacrimal and meibomian glands connected by the overlying tear film—plays a central role in vision. Molecular and cellular events involved in embryonic development, postnatal maturation, and maintenance of the ocular surface are precisely regulated at the level of gene expression by a well-coordinated network of transcription factors. A thorough appreciation of the biological characteristics of the ocular surface in terms of its gene expression profiles and their regulation provides us with a valuable insight into the pathophysiology of various blinding disorders that disrupt the normal development, maturation, and/or maintenance of the ocular surface. This paper summarizes the current status of our knowledge related to the ocular surface development and gene expression and the contribution of different transcription factors to this process.
The ocular surface consists of a continuous epithelial surface with regional specializations, including the surface and glandular epithelia of the cornea, conjunctiva, lacrimal gland, accessory lacrimal glands, and meibomian gland, which are connected by the overlying tear film (Figure 1). The ocular surface serves a pivotal role in vision by ensuring scatter-free transmission of light and appropriate refraction. The transparent and refractive functions of the avascular cornea are supported by the tear film produced by the surrounding tissues of the ocular surface. The protective tear film is a complex fluid consisting of an inner mesh of transmembrane mucins on the surface epithelial cells soaked in a central aqueous layer secreted by the lacrimal glands and the outermost lipid layer secreted by the meibomian glands. The aqueous layer contains soluble mucins secreted by the conjunctival goblet cells, and soluble proteins and solutes secreted by the surface epithelial cells and plays a major role in protective functions of the tear film. Defective development and/or maintenance of the ocular surface is a leading cause of vision-related problems. It is estimated that as many as 285 million people worldwide are visually impaired, of whom 39 million are blind due to a variety of reasons . Among them, corneal opacity accounts for about 4% of blindness, with trachoma causing another 3% .
Ocular surface development depends on a series of well-coordinated interactions between the neuroectoderm that forms the retina and the surface ectoderm that forms the lens and cornea, with important contributions from the neural crest-derived periocular mesenchymal cells (Figure 2) [2, 3]. One of the currently emerging themes in developmental ophthalmology is that the well-coordinated changes in gene expression accompanying ocular surface development are regulated by a combinatorial effect of a handful of transcription factors. This paper summarizes the current knowledge in regulation of gene expression during mouse ocular surface development.
2. Development, Maturation, and Maintenance of the Ocular Surface
The cornea comprises of the anterior epithelium (stratified squamous cells derived from the surface ectoderm), central stroma (a thick collagenous mass of extracellular matrix with scattered neural crest-derived keratocytes), and the posterior endothelium (a monolayer of neural crest-derived cells) (Figure 2) [4, 5]. In the mouse, early eye development begins with the formation of the lens placode around embryonic day 10 (E10) (Figure 2). The lens vesicle is formed around embryonic day 11 (E11) by invagination of the lens placode in response to signals from the underlying optic vesicle (Figure 2). The overlying head surface ectoderm forms the presumptive corneal epithelium, which remains 1-2 cell layered at birth. Following eyelid opening around postnatal (PN) day 12, 1-2 cell-layered corneal epithelial cells divide rapidly and differentiate to form the 5-6 cell-layered epithelium by PN21 and a mature 6–8 cell-layered stratified squamous epithelium by about 8–10 weeks after birth. Around E12.5, neural crest-derived mesenchymal cells that surround the developing eye begin to migrate in between the surface ectoderm and the lens vesicle, forming the presumptive stroma and the corneal endothelium by E15.5 (Figure 2). Note that the term corneal “endothelium” is a misnomer, as it consists of neural crest-derived cells rather than endodermis-derived cells. The corneal stromal cells produce and secrete an extracellular matrix comprising of collagen fibrils and a variety of proteoglycans, the precise organization of which ensures corneal curvature, transparence, and mechanical strength. In postnatal stages, the corneal stromal cell density decreases gradually. The monolayer of endothelial cells that line the posterior of the cornea form tight junctions and help maintain stromal hydration by pumping excess water from the stroma into the anterior chamber.
Though most features of the mature cornea are evident by about 6 weeks of age [4, 5], it is still debated as to when the fully mature cornea is formed. Proliferation and differentiation of the corneal epithelial cells continue in the adult mouse, allowing the sloughed-off superficial epithelial cells to be steadily replaced by differentiation of the slow-dividing basal cells, which in turn are replenished by stem cells originating from the corneal limbal epithelium [6–8]. The corneal epithelial cells accumulate high levels of taxon-specific corneal crystallins aldehyde dehydrogenase 3A1 (Aldh3a1) and transketolase (Tkt), which account for about 50% and 10% of water-soluble proteins, respectively [9–11], along with structural proteins such as keratin-12 . Though it is suggested that the corneal crystallins are essential for the transparent and refractive properties of the cornea, convincing evidence supporting such roles is still scanty .
A fully functional lacrimal system is in place by the time of mouse eyelid opening around PN12, when the cornea is first exposed to the environment. The mouse lacrimal gland formation begins with a bud-like invagination of the temporal conjunctival forniceal epithelium around E13, which starts branching around E15.5 . The meibomian gland buds, on the other hand, are apparent around E18.5 with ductal branching first detected at PN5 . Both FGF10 signaling and Pax6 play important roles in lacrimal gland formation [13, 15]. Branching and differentiation of both lacrimal and meibomian glands is complete by around eyelid opening. Conjunctival goblet cells, which produce and secrete soluble mucins to the tear film, also first appear around PN12 , meeting the final physiological requirement for a fully functional lacrimal system before the eyelids open.
3. Differentially Expressed Genes in Different Components of the Ocular Surface
Several attempts have been made to identify differentially expressed genes in the mature mouse cornea, conjunctiva, and the limbus located in between. The basal layer of limbal epithelium located between the conjunctiva and the cornea is enriched in stem cells that serve as a source for transient-amplifying cells which migrate to the central corneal epithelium. Comparison of the rat limbal and central corneal transcripts by serial analysis of gene expression (SAGE) identified 759 transcripts specific for the limbus and 844 transcripts specific for the central cornea, with 2292 transcripts present in both . A comparative analysis of the human corneal and conjunctival epithelial gene expression identified 93 and 211 transcripts exclusive to corneal and conjunctival epithelium, respectively. Biological processes related to cell adhesion, redox equilibria, and cytoprotection were overrepresented in the cornea, while innate immunity and melanogenesis were most prominent in the conjunctiva . Microarray analysis of the pig limbal side population cells (enriched in stem cells) identified the genes responsible for the slow cycling and low metabolic activity of the limbal stem cell population [19, 20]. In another study, laser capture microdissection followed by microarray analysis identified about 100 differentially expressed genes in the mouse limbal compared to corneal epithelial basal cells . Together, these studies identify differential gene expression profiles in these adjacent tissues and provide valuable insights related to the ocular surface cell biology.
Gene expression in the lacrimal glands has been the target of a few recent investigations. Large-scale sequencing of cDNA libraries generated from mouse and human lacrimal glands revealed significant differences suggestive of molecular divergence between the two species . Laser capture microdissection coupled with microarray analysis demonstrated polarized expression of transporters and channels in lacrimal gland duct cells, consistent with the relatively high K+ in lacrimal fluid . Differential gene expressions in the lacrimal gland during development of dry eye in a mouse model of Sjögren’s syndrome-like disease identified 552 differentially expressed genes, providing new insight into the underlying cause or regulation of Sjögren’s syndrome . Further analysis in these mice-revealed alterations in tight junctions, adherens junctions, desmosomes, and gap junctions, suggesting perturbations in the permeability of the paracellular spaces between epithelial barriers in Sjögren’s syndrome . Gene expression arrays using total RNA isolated from the human accessory lacrimal glands (also called Glands of Wolfring) which secrete directly onto the ocular surface collected from frozen sections of eyelids by laser microdissection identified 24 most highly expressed genes, many of which were of direct relevance to lacrimal function . The human accessory lacrimal glands are enriched in genes with antimicrobial activity and those related to protein synthesis and secretion, including ion channels and transporters, carbonic anhydrase, and aquaporins . Genes encoding lysozyme, lactoferrin, tear lipocalin, and lacritin were among the most highly expressed in the human accessory lacrimal glands .
Similar large-scale profiling of transcriptomes has been attempted in meibomian glands as well. A study designed to identify the changes in gene expression associated with human meibomian gland dysfunction (MGD) identified about 400 genes with significant alterations . In addition, this study revealed that the human meibomian gland gene expression signature is significantly different from that of the adjacent tissues . Comparison of gene expression in lacrimal and meibomian glands obtained from ovariectomized mice treated with testosterone, estrogen, or control vehicle for 14 days revealed the sex-specific effects of sex steroids in the lacrimal and meibomian glands [28, 29]. Taken together, these studies identify differential gene expression profiles in the ocular adnexa and reveal the molecular basis for complex pathophysiological responses in tear film composition to sex hormones and during MGD.
4. Gene Expression during Corneal Development
Most large-scale studies of gene expression in corneas have attempted to characterize the early postnatal or adult mouse or rat corneal transcriptomes. No large-scale study has addressed the changes in gene expression patterns during human or mouse corneal embryonic development or in human corneas with developmental defects or diseases, presumably due to scarcity of tissues. Considering the important changes in mouse corneal morphology during post-eyelid-opening stages described above, a few studies have characterized the accompanying changes in corneal gene expression. Microarray analysis of using Affymetrix MG74Av2 chips targeting 8,666 unique characterized genes identified 442 genes differentially expressed between immature (PN10) and adult (PN49 to PN56) groups . In a more thorough analysis, comparison of PN9 and 6-week-old adult mouse corneas by serial analysis of gene expression (SAGE) revealed dynamic changes in gene expression accompanying corneal postnatal maturation . Roughly one third of the transcripts expressed in the PN9 or mature corneas were determined to be exclusive to each stage, with the remaining one third expressed at both stages .
By comparing the WT corneal transcriptomes at PN11 and PN56, we identified 1574 and 1915 genes whose expression decreased and increased, respectively, by more than 2-fold between PN11 and PN56 . Apart from validating the previously identified changes in gene expression during corneal maturation [30, 31], this study identified additional genes whose expression was modulated in the cornea following eyelid opening. Transcripts encoding major extracellular matrix-(ECM-) related proteins were decreased between PN11 and PN56, suggesting that most of the stromal ECM is produced before or around eyelid opening. Consistent with the limited remodeling that occurs in the adult corneal stroma , Adam family proteinases and other MMPs required for remodeling ECM  were sharply decreased between PN11 and PN56. Expression of several cell-junctional complex components increased between PN11 and PN56, when the corneal epithelium stratifies. Similarly, several oxidative stress-related genes and solute carrier family members were upregulated between PN11 and PN56, reflecting the elevated oxidative stress and the need for solute transport in metabolically active adult corneas .
5. Gene Expression at the Corneal Limbus
The human corneal limbal epithelial crypt, an approximately 120 μm long cord of cells, arises from the undersurface of interpalisade rete ridges of the palisades of Vogt in the corneoscleral limbus. Each human eye contains from 6 to 7 such limbal epithelial crypts randomly distributed along the limbus. It is largely accepted that the normal corneal epithelial homeostasis including epithelial wound healing and repopulating the cells sloughed off at the surface depends on normal division, migration, and stratification of the corneal epithelial stem cells that reside in the limbal epithelial crypts [35, 36]. A recent study challenged the notion that stem cells are limited to the corneal limbal area by demonstrating the presence of oligopotent stem cells dispersed throughout the cornea . It is possible that these central corneal epithelial stem cells facilitate routine maintenance of the corneal epithelium while the limbal stem cells step in to repair acutely damaged corneal epithelium. In spite of this report, the clinical importance of corneal limbal stem cells is well established as judged by the successful use of limbal epithelial stem cell-derived cell sheaths in treating corneal defects [38–43]. Considering that the limbal epithelial stem cells serve as a useful resource for reconstruction of defective corneas, thorough understanding of the stem cell-enriched limbal epithelial gene expression patterns is necessary to create new opportunities for diagnostic and therapeutic interventions in severely damaged corneas .
In spite of their importance, identification of corneal limbal stem cell-specific markers has been a daunting challenge. Comparison of the rat limbal and central corneal epithelial transcriptomes by SAGE identified 759 limbal specific transcripts, 844 central corneal specific transcripts, and 2292 transcripts that were present in both regions . Microarray comparison of transcriptomes from laser-microdissected limbal and central corneal basal epithelial cells revealed the identities of about 100 genes that were specifically expressed in the corneal limbus . Microarray comparison of transcripts in the human limbal epithelial crypts with those in the cornea identified genes involved in cell cycling and self-renewal such as growth factors, cytokines, WNT, Notch, TGF-Beta pathways FZD7, BTG1, CCNG, and STAT3 as enriched in the cornea . In contrast, genes involved in stem cell maintenance, such as cell adhesion molecules, WNT, and Notch-signaling pathway components, CDH1, SERPINF1, LEF1, FRZB1, KRT19, SOD2, and EGR1, were overexpressed in the limbal crypt . Mitotically quiescent limbal stem cells may be identified by their coexpression of CCAAT enhancer binding protein-delta (C/EBPδ), Bmi1, and δNp63α . In addition, genes, including Krt15, Krt14, N-cadherin, cadherin-3, nestin, SOD2, Wnt4, Notch-1, SPON1, IFITM1, ITM2A, CXCR4, DKK4, NGF, and its receptor TrkA, have been proposed as corneal limbal basal epithelial stem cell-specific markers [46–52]. Wnt/β-catenin signaling is thought to regulate human corneal epithelial stem/progenitor cell proliferation . Though a direct role has been proposed for transcription factors Pax6, δNp63α, EGR1, TCF4, and C/EBPδ in maintenance of corneal limbal stem cell identity, involvement of other transcription factors controlling the limbal epithelial cell fate remains to be identified [45, 54, 55]. Though generally useful, these studies have failed to identify specific markers which could be used to define the corneal limbal stem cells at the molecular level. We are yet to arrive at a consensus regarding definitive markers for corneal limbal epithelial stem cells; the presence of which was first proposed more than two decades ago [56, 57].
6. Gene Expression in the Conjunctiva
Unlike the cornea, relatively few studies have addressed the changes in gene expression in the conjunctiva (Figure 3). In addition to the epithelial cells, conjunctiva contains goblet cells which produce and secrete mucins to the tear film. Conjunctival goblet cells play an important role in maintaining ocular surface homeostasis by producing and secreting mucins to the tear film . In spite of their importance, relatively little is known about the factors regulating conjunctival goblet cells. Studies in other tissues have demonstrated the requirement of factors such as Foxa1, Foxa2, Foxa3, and Spdef for colonic and airway epithelial goblet cell development [59–63]. Studies in our laboratory have demonstrated that Klf4 and Klf5 are both required for conjunctival goblet cell development (Figure 3) [64, 65].
In order to identify the changes in postnatal mouse conjunctival forniceal gene expression and their regulation by Klf4 during the eye-opening stage when the goblet cells first appear, we used Laser microdissection (LMD) to collect conjunctival forniceal cells from PN 9, PN14, and PN20 wildtype (WT) and PN14 Klf4-conditional null (Klf4CN) mice, in which goblet cells are absent, developing, present, and missing, respectively. We identified 668, 251, 1160, and 139 transcripts that were increased and 492, 377, 1419, and 57 transcripts that were decreased between PN9 and PN14, PN14 and PN20, PN9 and PN20, and PN14 WT and Klf4CN conjunctiva, respectively . Comparison of the conjunctival Klf4-target genes  with the corneal Klf4-target genes  identified a small number of common target genes, suggesting that Klf4 performs distinct functions in different tissues, by regulating a diverse array of targets. This tissue-selective nature of Klf4 is important in view of the widespread expression of Klf4 in several parts of the body. How such tissue-selective nature is achieved remains to be understood.
Klf4 may exert its influence on conjunctival goblet cells directly or by controlling the expression of other transcription factors regulating goblet cell development. By comparing the wild type and Klf4CN PN14 conjunctival forniceal gene expression, we identified the transcription factors affected in the Klf4CN conjunctiva. Four among these factors were previously shown to be required for goblet cell development in other tissues such as colon or lung. Transcripts encoding Spdef, Foxa1, and Foxa3 that regulate goblet cell development and epithelium-specific Ets (ESE) transcription factor family members were increased during conjunctival development .
Sterile alpha motif- (SAM-) pointed domain containing Ets family protein (SPDEF) is a member of the ETS family of transcription factors. SPDEF contains an ETS DNA-binding domain at the C-terminal and a regulatory region consisting of the SAM-pointed domain at the N-terminal . It preferentially interacts with ETS-binding sites with a core sequence GGAT. Spdef is required for goblet cell development in the intestine [63, 67] and tracheal/laryngeal submucosal glands as well as the conducting airway epithelium after allergen exposure [61, 62]. In transient transfection assays, both Klf4 and Klf5 stimulated mouse Spdef promoter activity (Gupta and Swamynathan, unpublished). However, Klf5 had a relatively greater effect, and cotransfection with Klf4 and Klf5 did not have any additional stimulatory effect, suggesting that Klf4 and Klf5 act through the same cis-elements in Spdef promoter (Gupta and Swamynathan, unpublished) (Figure 3).
Though the studies summarized above have given us a general picture of the goblet cell gene expression patterns, we are yet to understand what goes wrong in pathophysiological conditions which affect goblet cell densities. For example, changes in gene expression associated with goblet cell hyperplasia in allergic conjunctivitis and asthma or, alternatively, their absence in ocular cicatrizing pemphigoids remains to be identified. Additional studies in these directions are necessary to gain a better understanding of the genetic network of transcription factors which regulate goblet cell development and function in healthy and disease conditions.
7. MicroRNAs in the Ocular Surface
Endogenous noncoding microRNAs (miRNAs) regulate development and differentiation by binding to complementary sequences within the 3′ untranslated region (UTR) of target mRNAs, affecting the stability of target mRNAs and modulating their translation . About 30% of the protein-coding genes in the vertebrate genome are estimated to be regulated by miRNAs. One miRNA can target hundreds of target mRNAs, and a given mRNA can be targeted by multiple miRNAs, resulting in increased complexity of gene regulation by miRNAs. In the eye, several miRNAs expressed in a distinct tissue and cell-type specific manner have been detected [69–71]. Most of the studies on miRNAs in the ocular surface are focused on the cornea. MiRNAs expressed in and important for the rest of the ocular surface remain to be examined.
In a comprehensive survey of miRNA expression in ocular tissues using microarray and RNA in situ hybridization, different ocular tissues exhibited notably distinct miRNA enrichment patterns . Cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues. Targeted disruption of Dicer, a ribonuclease essential for miRNA processing, disrupted corneal epithelium stratification and whole eye development, providing evidence for the importance of miRNAs in eye development .
MiR184, one of the most well-studied miRNAs in the cornea, is abundantly expressed in the corneal epithelium. A mutation in miR-184 is responsible for familial keratoconus, a severe and painful corneal disorder . Expression of miR-184 is detected in early eye development and corneal epithelial differentiation of human-induced pluripotent stem cells (hiPSCs) . The knockdown of miR-184 resulted in a decrease in Pax6 and keratin-3, consistent with the observation that a point mutation in miR-184 results in corneal dystrophy . In the first example of an miRNA negatively regulating another to maintain the levels of a target protein, the corneal epithelial-specific miR-184 was found to antagonize miR-205 that is widely expressed in the anterior segment epithelia and epidermis [69, 75]. miR-184 interferes with the ability of miR-205 to suppress the levels of lipid phosphatase SHIP2, thus maintaining its proper levels in the corneal epithelium .
Many other miRNAs play important roles in corneal development. Among them is miR-145, which regulates corneal epithelium formation and maintenance of epithelial integrity, by targeting the expression of integrin ITGB8 . Another miRNA, miR-450b-5p, was identified as a molecular switch for Pax6 . MiR-450b-5p and Pax6 are reciprocally distributed at the presumptive epidermis and ocular surface, respectively. MiR-450b-5p inhibited Pax6 expression and corneal epithelial fate in vitro, suggesting that miR-450b-5p triggers epidermal specification of the ectoderm by repressing Pax6. Thus, the absence of miR-450b-5p allows ocular epithelial development .
8. Transcription Factors Regulating Ocular Surface Development
Developmental studies utilizing transgenic and knockout technologies have revealed the contributions of a number of different transcription factors to mouse eye development (Figure 4). Human ocular surface developmental disorders associated with defects in genes encoding these transcription factors are consistent with their conserved roles in eye development across species. A brief review of the contribution of these transcription factors to maturation and maintenance of the ocular surface is provided below.
8.1. Homeobox Transcription Factors
8.1.1. Paired Domain-Homeodomain Transcription Factor Pax6
Pax6, a paired domain-homeobox transcription factor, is considered the master regulator of eye development in view of its pivotal, highly conserved role in morphogenesis of the eye . In the mouse, Pax6 expression is first detected at the optic pit, head surface ectoderm, and neural ectoderm on E8. After E13.5, Pax6 is expressed in the proliferating anterior epithelial cells of the lens vesicle and the surface ectoderm which give rise to cornea, conjunctiva, and eyelids. The early embryonic expression of Pax6 in the developing eye is autoregulated by Pax6 and, in addition, by other homeobox transcription factors such as Meis1 and Meis2 (Figure 4) . Pax6 activity is required in the lens primordium for lens formation and for correct placement of the retina in the eye . In addition, Pax6 is expressed throughout the ocular surface , where it plays a major role in early embryonic development and postnatal maturation of the corneal and conjunctival epithelia [81–83]. Though Pax6 is required for lacrimal gland development , its involvement in meibomian gland development is yet to be explored. In the adult mouse, Pax6 continues to be expressed in the lens epithelial cells, cornea, conjunctiva, iris, ciliary body, and retina  and plays a major role in maintenance of the ocular surface.
Pax6 is required for early embryonic development of the corneal epithelium, stroma, and endothelium [82, 83] as well as corneal innervation . Mutations in Pax6 result in severe defects in the human eye anterior segment [85–88]. Homozygous Pax6 mutant mice develop only rudiments of the optic vesicle and die in the neonatal stage . Corneal abnormalities in the heterozygous Pax6 (Small eye, Sey) mouse mimic human aniridia-related keratopathy . The lens placode formation is delayed in Pax6 heterozygous mice, resulting in a smaller lens frequently fused to the cornea, resembling Peter’s anomaly . The corneal epithelium in Pax6+/− (Sey) mouse is thinner with reduced number of cell layers despite increased cell proliferation, suggestive of increased epithelial erosion. The Sey mouse corneal epithelium contains decreased levels of desmoglein, β-catenin, γ-catenin, and keratin-12, consistent with defective intercellular adhesion . In addition, the Sey mouse corneal epithelial cells have defective cell surface glycoconjugates that restrict their ability to migrate during wound healing . Distribution of neural crest-derived cells is abnormal in Sey mouse, indicating that Pax6 regulates the normal distribution and integration of neural crest-derived cells in the mouse cornea .
Corneal activities of Pax6 are dosage dependent, as shown by the defective morphogenesis when Pax6 is either over- or underexpressed . Overexpression of Pax6 in the mouse cornea affected corneal epithelial cell proliferation and homeostasis, resulting in signs of inflammation and neovascularization [95, 96]. An interaction between Pax6 dosage and hedgehog signaling is necessary for maintenance and regeneration of the corneal epithelium . Aberrant Pax6 dosage results in an abnormal corneal stroma and endothelium as well, suggesting that proper Pax6 dosage is essential for normal morphogenesis of all layers of the mouse cornea .
Pax6 influences ocular surface development both directly by controlling the expression of different genes that play critical roles in the ocular surface and indirectly by controlling many other transcription factors including Six3, c-Maf, MafA/L-Maf, Prox1, Sox2, and Foxe3 that play important roles in the ocular surface (Figure 4) [3, 79, 99–105]. Compound heterozygous mice with mutations in both Pax6 and Gli3 develop more severe phenotype than Gli3+/− or Pax6+/− mutants alone , providing evidence for functional interaction of Pax6 with other transcription factors. Pax6 regulates the expression of δNp63, a transcription factor which in turn regulates limbal epithelial stem cell (LESC) proliferation . Pax6 regulates the expression of Six3, a homeobox transcription factor that influences eye development directly by regulating the expression of structural and metabolic genes required for eye formation and indirectly by reciprocally activating the expression of Pax6 [107–110].
(1) Contributions of Pax6 towards Maintenance of the Mature Cornea. Maintenance of the mature corneal epithelium involves continuous replication of a slow dividing population of stem cells that resides in specialized niche areas near palisades of Vogt in the corneoscleral junction called limbus . The transiently amplifying cells derived from the LESCs differentiate as they migrate centripetally and apically and are eventually sloughed off at the surface [5, 35, 111]. Pax6 plays an important role in mature corneal homeostasis by helping maintain the limbal epithelial stem cell population. The limbal stem cells deficiency (LSCD) results in pterygium (characterized by in-growth of conjunctival cells and corneal neovascularization eventually resulting in corneal opacity) . Similarly, LSCD is thought to cause aniridia, linked with human PAX6 gene mutations. The pathophysiology associated with aniridia-related keratopathy (ARK) is likely caused by LSCD, with associated defects in wound healing responses [112, 113].
Pax6 plays a critical role in adult corneal epithelial wound healing as well [94, 112]. Pax6+/− mice display many defects in corneal cell surface glycoconjugates and wound healing . Pax6 influences corneal epithelial wound healing in association with hedgehog signaling . Pax6 is elevated at the migrating wound epithelial edge where it upregulates gelatinase B (gelB; MMP-9) . Pax6 influences target gene expression both independently and in association with other transcription factors such as pRb, MafA, MitF, Sox2, AP2α, and Sox3. Sox2 and Sox3 interact with Pax6, leading to synergistic transcriptional activation . There are two Pax6 binding sites within the gelB −522/+19 bp promoter fragment . Pax6 controls the gelB promoter activity by interacting directly with one of these sites and indirectly with the other site, through cooperative interactions with AP2α . Overall, these studies indicate that Pax6 plays a significant role in embryonic development, postnatal maturation and maintenance of cornea.
8.1.2. POU Homeodomain Transcription Factor Pitx2
Bicoid-related POU homeodomain transcription factor Pitx2, expressed in the neural crest, and the mesoderm-derived precursors of the periocular mesenchyme also contribute to the ocular surface development. In humans, PITX2 mutations account for a large portion of the Axenfeld-Rieger malformations of the anterior segment . In the mouse, deletion of Pitx2 resulted in severe disruption of periocular mesenchyme structures and extrinsic defects in early optic nerve development. Pitx2 is required in neural crest for specification of the corneal endothelium and stroma and the sclera . Corneal functions of Pitx2 also appear to be dosage dependent. Pitx2 heterozygous mutant mice display reduced central corneal thickness , while overexpression of Pitx2a isoform in the mouse corneal mesenchyme and iris results in corneal opacification, corneal hypertrophy, and iridocorneal adhesions .
Pitx2 influences eye development through its involvement in retinoic acid signaling and Wnt signaling pathways which play integral roles in the periocular mesenchyme. Pitx2 activates Dkk2, an antagonist of canonical Wnt signaling, suppressing canonical Wnt pathway during eye development . Retinoic acid signaling indirectly represses Wnt signaling in perioptic mesenchyme via induction of Pitx2 . Thus, Pitx2 regulates early eye development by serving as a link in the crosstalk between retinoic acid signaling and Wnt signaling. The anterior segment expression of Pitx2 is regulated by orphan G-protein-coupled receptor 48- (Gpr48/LGR4-) mediated cAMP-CREB signaling pathway .
8.2. Nonhomeobox Transcription Factors Regulating the Development of Cornea
8.2.1. High Mobility Group Protein Hmgn1
The nucleosome binding high mobility group (HMGN) proteins are a group of nonhistone nuclear proteins that influence gene expression by altering the chromatin structure. Many different members of the Hmgn family proteins are expressed in the developing eye . The Hmgn1-null mice develop thin, poorly stratified corneal epithelium depleted of suprabasal wing cells and a disorganized basement membrane, suggesting that Hmgn1 plays essential roles during mouse corneal epithelial development . Epithelial cell-specific markers glutathione S-transferase- (GST-) α4 and -ο1 are reduced in Hmgn1-null corneas, while the components of adherens junctions—E-cadherin and α-, β-, and γ-catenin—are upregulated .
8.2.2. Winged Helix/Forkhead Transcription Factors
Forkhead box (FOX) proteins are a family of transcription factors that share homology with the forkhead transcription factor in Drosophila  and play important roles in embryonic development by regulating the expression of genes involved in cell growth, proliferation, and differentiation [125, 126]. A unified nomenclature grouped the FOX proteins into subclasses (FOXA-FOXS) based on sequence conservation . Many forkhead family members play important roles in normal development of different components of the ocular surface [59, 60, 128–140]. Among them, Foxc1 and Foxc2 have attracted the most attention in view of their association with Axenfeld-Rieger syndrome (ARS).
Foxc1 is the first forkhead factor to be associated with ocular surface development . The expression of Foxc1 gene is first detected in the periocular mesenchyme at E11.5 and is downregulated as the corneal endothelium differentiates . Foxc1-null mice die at birth with multiple abnormalities including anterior segment dysgenesis involving corneolenticular fusion with a thicker corneal epithelium, disorganized stroma, and missing endothelium [131, 133, 141]. Foxc1 heterozygous mice are viable with milder anterior segment defects [131, 139]. In humans, autosomal dominant mutations in FOXC1 gene (in addition to those described above for PITX2) have been documented to cause anterior segment dysgenesis resembling ARS which affects additional parts of the body including the teeth and abdominal region [132, 142–145].
Corneal avascularity is an essential feature that ensures unimpeded transmission of light towards the retina. Recent studies have begun to unravel the well-conserved mechanisms that ensure corneal angiogenic privilege [146, 147]. Foxc1 plays an integral role in this regulation by controlling the normal delicate balance between factors in the cornea that promote angiogenesis and those that inhibit it. Interestingly, though Foxc1 is required for preserving mouse corneal transparency by inhibiting vascular growth , studies in human patients with FOXC1 mutations contradict this finding , suggesting species-specific functions for Foxc1.
Foxc1 and Foxc2 have nearly identical DNA binding domains, expression patterns, and functions in the developing eye [130, 139]. Mutations in FOXC2 also resulted in ocular anterior segment anomalies, suggesting overlapping functions for these related factors [128, 134]. Foxc1 and Foxc2 double heterozygous mice have malformations of the ciliary body not seen in either heterozygous mouse alone . Overlapping influence of forkhead family transcription factors FOXC1 and FOXC2 and the POU domain factor PITX2 described above may explain the variability and heterogeneity associated with the anterior segment dysgenesis in ARS [149, 150].
Forkhead box transcription factors also play a significant role in the development of other compartments of the ocular surface. Foxc1 is expressed in both the epithelium of the lacrimal gland and the surrounding mesenchyme. Foxc1 influences lacrimal gland development, as demonstrated by the severely impaired lacrimal glands in homozygous null Foxc1 mouse mutants with reduced outgrowth and branching .
Other related forkhead box transcription factors also influence anterior segment morphogenesis. For example, Foxe3 is required for anterior segment morphogenesis and differentiation in a Pax6 gene dosage-dependent manner . Mutations in Foxe3 are associated with defective lens development, and iridocorneal and iridolenticular fusions reminiscent of Peter’s anomaly . Foxe3 expression in the eye is regulated by Msx2, a transcription factor implicated in anterior segment development . A recent study identified a novel forkhead factor Foxf2, located near the Foxc1 locus, as another candidate factor regulating anterior segment morphogenesis . Heterozygote Foxf2 mutant mice display thinner iris, hyperplasia of the trabecular meshwork, small or absent Schlemm’s canal, and a smaller iridocorneal angle, while homozygous Foxf2 mutant pups lack ciliary body projections at E18.5, suggesting a dosage-dependent role for Foxf2 in anterior segment morphogenesis .
8.2.3. Sp1/Krüppel-Like Transcription Factors
Sp1/Krüppel-like transcription factors belong to the large family of zinc finger family proteins . Several members of this family are expressed in the ocular surface in an overlapping manner [31, 153]. Members of the Sp1/KLF family possess divergent regulatory domains but similar DNA-binding domains, which enable them to bind similar cis-elements with comparable affinity, allowing fine regulation of their target genes in response to different stimuli. Here, I summarize the roles of Sp1/KLFs in ocular surface development and gene expression.
(1) Specificity Protein Sp1. Sp1 expression first detected in the head surface ectoderm as early as E10.5 continues in the cornea in the later stages . However, Sp1 levels in the cornea decline gradually following eyelid opening. Within the cornea, Sp1 appears to be relatively more abundant in the basal cell layers and keratocytes. Expression of Sp1 is upregulated in a severe cornea-thinning disease called keratoconus [155, 156]. Sp1 is believed to play a role in keratoconus disease progression by supporting the increased expression of degradative enzymes such as cathepsin-B and suppression of proteinase inhibitors such as α1-proteinase inhibitor (α1-PI) [156, 157]. Downregulation of α1-PI in the corneal epithelium mediated by Sp1 may be a key event in keratoconus progression, supporting the possibility that the corneal epithelium also is involved in keratoconus, along with the stroma .
Sp1 is involved in regulation of several genes with important functions in the cornea. For example, Sp1 regulates corneal expression of keratin-3 in association with AP-2 [159, 160] and keratin-4 promoter activity in a cyclin D1-regulated manner . Sp1 also activates corneal epithelial involucrin gene expression . A recent study demonstrated that Sp1 activates expression of α5-integrin in association with another transcription factor, AP-1 . Expression of Sp1 is elevated during wound healing, where it may be required for elevated expression of gelatinase-B (MMP9) .
(2) Krüppel-Like Factor 4 (Klf4). Krüppel-like transcription factor Klf4 is one of the most highly expressed transcription factors in the mouse cornea . Klf4 is one of the four transcription factors (along with Oct3/4, Sox2, and c-Myc) required for generation of induced pluripotent stem cells from mouse embryonic or adult fibroblasts . As each of these factors is present in the corneal limbus, it is likely that they are involved in the maintenance of limbal stem cells, the source of epithelial cells in the mature cornea.
Klf4 expression is detected in the ocular surface around E10 and is sustained in the adult cornea, increasing after eyelid opening. Conditional disruption of Klf4 in the surface ectoderm-derived structures of the eye resulted in fragile corneal epithelium, swollen, vacuolated basal epithelial and endothelial cells, edematous stroma, and loss of conjunctival goblet cells . Interestingly, Klf4 is also required for colonic goblet cell development , suggesting that similar networks may regulate goblet cell development in diverse mucosal epithelia. Stromal edema in Klf4CN corneas is associated with defective collagen fibril organization and increased degradation of stromal proteoglycans . Klf4CN corneal stromal edema and epithelial fragility coupled with hypercellularity in these stromas suggested a proinflammatory environment in Klf4CN corneas . However, the identity of these infiltrating cells remains to be established.
Studies using germline or conditional knockout mice demonstrated that Klf4 is required for both skin and corneal epithelial barrier function through regulation of expression of different components of the desmosomes [168, 169]. Direct involvement of Klf4 has been demonstrated in regulation of corneal epithelial expression of keratin-12, aquaporin-3, aquaporin-5, and corneal crystallins TKT and Aldh3a1 [65, 66]. Microarray comparison of WT and Klf4CN corneal and conjunctival gene expression patterns helped identify the potential Klf4-target genes in these two adjacent tissues of the ocular surface [16, 66]. Significant differences in conjunctival and corneal Klf4-target genes suggested tissue-dependent regulatory targets for Klf4 [16, 66].
(3) Krüppel-Like Factor 5 (Klf5). Klf5 is structurally related to Klf4 with an identical C-terminal DNA-binding domain capable of binding similar cis-elements and a divergent N-terminal regulatory domain that allows them to exert divergent influence on their target promoters . Klf5 expression largely overlaps with that of Klf4, raising interesting questions related to their choice of target genes in a tissue where they are coexpressed. The ocular surface expression of Klf5, detected as early as E12, increases during postnatal stages . Following apparently normal embryonic eye morphogenesis, Klf5CN corneas displayed defective postnatal maturation resulting in relatively smaller eyes containing translucent corneas with fragile epithelium, abnormal epithelial basement membrane, and edematous and hypercellular stroma . In addition, Klf5CN eyelids were malformed with defective meibomian glands. Klf5CN conjunctiva lacked goblet cells, suggesting that Klf5 is required for conjunctival goblet cell development. Klf5 is also required for lung goblet cell development, suggesting that similar transcriptional networks regulate goblet cell development in diverse mucosal epithelia [64, 170].
Severe phenotype observed when either Klf4 or Klf5 was disrupted in the ocular surface, keeping the other gene intact, suggested nonredundant functions for these two structurally related factors. Consistent with this, simultaneous co-ablation of both Klf4 and Klf5 resulted in a more severe ocular surface phenotype compared with Klf4CN or Klf5CN, demonstrating that Klf4 and Klf5 share few, if any, redundant functions . Finally, comparison of corneal Klf4- and Klf5-target genes revealed that roughly one-third of the target genes are unique to each factor, with the other third being shared, common target genes .
(4) Krüppel Like Factor 6 (Klf6). Klf6 expression is detected in the head surface ectoderm as early as E10 and in the corneal epithelium and stroma around E15.5, and it increases in postnatal stages . Klf6 also binds and activates keratin-12 (Krt12), an intermediate filament required for corneal epithelial homeostasis . However, it is not clear if Klf4 and Klf6 activate Krt12 through the same cis-elements, or if they target different regions of the Krt12 promoter. Interestingly, KLF6 expression also is elevated in keratoconus, a progressive disease associated with thinning and scarring of the cornea . A likely explanation for the involvement of KLF6 in keratoconus may be found in the fact that KLF6, like Sp1 described above, downregulates the α1-proteinase inhibitor (α1-PI) gene in corneal epithelial cells .
8.2.4. Activating Protein (AP) Family
(1) Activating Protein-1 (AP1). AP1 consists of a group of basic leucine zipper (bZip) family of dimeric complexes formed by the various Jun, Fos, Fra, and ATF proteins. Members of the AP1 family selectively bind the tetradecanoylphorbol acetate- (TPA-) responsive element (TRE; 5′-TGAG/CTCA-3′) and activate transcription of nearby promoters. AP1 family members regulate cell proliferation in response to various stimuli. Many AP1 family members are expressed in the cornea (Figure 4) [31, 174–177]. AP1 is necessary for expression of involucrin, a structural protein that is selectively expressed in differentiating corneal epithelial cells [162, 177]. In transgenic mice, removal of the AP1 site by truncation or point mutation results in a loss of involucrin expression, confirming the importance of AP1 for involucrin promoter activity during corneal epithelial cell differentiation.
(2) Activating Protein-2 (AP-2). The activating protein-2 (AP-2) family of transcription factors, consisting of five different members AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε, stimulate proliferation and suppress terminal differentiation in a cell-type-specific manner during embryonic development . Corneal epithelial expression of AP-2α is confined to basal epithelial cells while AP-2β is broadly expressed throughout all cell layers [179–181]. AP-2α is also highly expressed in the less differentiated cell layers of the eyelid epidermis . Ap-2α null embryos exhibit a range of phenotypes from a complete lack of eyes to defective lens attached to the overlying surface ectoderm . Conditional deletion of Ap-2α in lens placode derivatives, including the corneal epithelium, results in a decrease in the expression of the cell-cell adhesion molecule E-cadherin, misexpression of laminin, entactin and type IV collagen, and disruption of stromal collagen fibril organization, showing that AP-2α is required for proper formation of the mouse cornea [179, 182]. Pax6 and AP-2α interact with each other and coordinate the expression of gelatinase-B (matrix metalloproteinase 9) and corneal epithelial repair .
8.2.5. Ets Family Members
The Ets transcription factors belong to a large family comprising of 29 related genes in humans (28 in the mouse) named after E-twenty six (E26), a gene transduced by the leukemia virus. Ets family members are expressed throughout the body and are implicated in development and cancer progression. Ets family transcription factors are involved in regulation of a wide variety of functions including cell cycle control, cell differentiation, cell migration, cell proliferation, apoptosis, and angiogenesis. Ets family transcription factors are characterized by a conserved winged helix-turn-helix DNA-binding domain that binds the consensus DNA sequence 5′-GGA(A/T)-3′. Additional sequence specificity is achieved through interaction with other cofactors and the neighboring sequence. Ets family members often influence gene expression in cooperation with other transcription factors, which make their effects more versatile. For example, Ets-1 and Ets-2 cooperate with the AP-1 transcription factor, while Elk-1 and SAP-1 cooperate with the serum response factor (SRF).
The protooncogene Ets-1, which plays a key role in angiogenesis and matrix degradation, is upregulated in many cases of pterygial angiogenesis . An Ets family epithelium-specific transcription factor Ese-1/Elf3 is upregulated in differentiating mouse corneal epithelium and in immortalized human corneal epithelial (HCE) cells, and transactivates keratin-12 through Ets-binding sites . Suppression of Ese-1/Elf3 expression by antisense RNA in HCE cells affects their differentiation, providing evidence for the involvement of Ese-1/Elf3 in differentiation of corneal epithelial cells (Figure 4).
8.2.6. Transcription Factors Regulating Hypoxic Stress Response in the Cornea
When the eyelids are closed during sleep, the avascular cornea is subjected to almost 75% drop in oxygen partial pressure [185, 186]. Thus, hypoxic and xenobiotic response pathways are essential for proper maintenance of corneal homeostasis. The important role of inhibitory PAS (IPAS) domain protein—a hypoxia repressor protein—in maintaining corneal avascularity [187, 188] is consistent with this prediction. IPAS gene expression is stimulated by hypoxia inducible factor-1α (HIF-1α) demonstrating a negative feedback regulatory circuit . Additional evidence for the involvement of hypoxic and xenobiotic stress in regulating corneal gene expression comes from the fact that corneal crystallin genes are induced by hypoxia or xenobiotics [189–191]. We demonstrated that the xenobiotic metabolism-related pathways are significantly enriched among genes whose expression is decreased in Klf5CN corneas, suggesting that Klf5 serves an important role in detoxification of the environmentally exposed avascular cornea . Other transcription factors such as NF-κB, Klf5, Cited2, and CTCF are implicated in regulating hypoxia-related gene expression and are discussed below.
(1) NF-κB and the Related Factors. NF-κB plays an essential role in corneal wound healing process. NF-κB is up-regulated in the early stage of cauterization induced corneal neovascularization (CNV), suggesting that it participates in wound healing, inflammation, and neovascularization in the cornea . NF-κB is activated in corneal pathologies involving increased plasma levels of LPS and Tumor Necrosis Factor-α (TNF-α), as well as direct UV-B exposure . TNF-α disrupted the barrier function of cultured human corneal epithelial cells in an NF-κB-dependent manner . Thymosin-β4 suppresses NF-κB activity, providing evidence suggesting that its anti-inflammatory effects are mediated through NF-κB [195, 196].
NF-κB plays important roles in resolving corneal viral infections. Herpes simplex virus- (HSV-) 1 infection of the human cornea first induces and then blocks epithelial cell apoptosis in an NF-κB-dependent manner . NF-κB is activated in respiratory syncitial virus-infected corneal epithelial cells . NF-κB activation is partly responsible for the acute inflammation in adenoviral-infected corneas . NF-κB and PI3K-Akt signaling pathways mediate the poly(I:C-) induced VCAM-1 and ICAM-1 upregulation in corneal fibroblasts, thus regulating the corneal stroma inflammatory responses to viral infection .
NF-κB pathway is rather complex and is regulated by several inhibitors, which also play a role in regulating corneal homeostasis. I-κKα is required for formation of cornea and conjunctiva, possibly due to its ability to regulate NF-κB activity . I-κB-ζ is another regulator of the transcription factor NF-κB and is expressed in the ocular surface epithelium, a part of the mucosal defense system . The pathologic progression of ocular surface inflammation is inhibited by I-κB-ζ , as demonstrated by the chronic inflammation in I-κB-ζ-null mouse ocular surface accompanied by loss of conjunctival goblet cells .
(2) Cited2. Cited2 is an acronym for “Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain-2.” Cited2 is a transcriptional coactivator in the p300/CBP-mediated transcription complex. It acts as a bridge linking p300/CBP transcriptional coactivator complex with TFAP2 transcription factors, stimulating TFAP2-target gene promoter activities. Cited2 acts as a positive regulator of TGF-β signaling through SMAD/p300/CBP-mediated transcriptional coactivator complex. CITED2 ectopic expression attenuated the expression of NFκB-responsive genes, while CITED2 knockdown stimulated the NFκB-responsive genes, suggesting that Cited2 acts as a negative regulator of NFκB . Mechanistic basis of this action was revealed by the discovery that CITED2 prevented p300-mediated acetylation of p65 subunit of NFκB, thus attenuating p65 binding to its cognate promoters .
Conditional disruption of Cited2 resulted in severe ocular surface defects including corneal opacity and spontaneous corneal neovascularization at older age . In the absence of Cited2, there were fewer layers of corneal epithelial cells that lacked Krt12 expression. Cited2 deficient corneal epithelial debridement wound healing was impaired. Additional gene expression studies indicated that Cited2 may influence corneal morphogenesis by modulating the expression of Pax6 and Klf4 . CITED2 negatively regulates the expression of hypoxia-responsive genes. CITED2 serves as a negative regulator of hypoxia-inducible factor-1 (HIF-1) by interacting with the p300/CBP CH1 domain, disrupting the formation of the HIF-1 and p300/CREB-binding protein (p300/CBP) heterodimeric complex .
(3) CCCTC-Binding Factor (CTCF). CTCF is an epigenetic transcription factor containing a 11-zinc finger DNA-binding domain . CTCF is considered a key player in insulator function, which isolates the effects of cis-elements from spreading beyond the intended loci in eukaryotic genomes. The presence of 11 zinc fingers provides this factor with an ability to interact with a host of different target sites, increasing its functional versatility .
CTCF is believed to play a role in epidermal growth factor-(EGF-) mediated suppression of Pax6 accompanying elevated cell proliferation in corneal epithelial cells . Overexpression of CTCF suppressed Pax6 P0 promoter activity through CTCF binding elements located around −1.2 kb upstream of the P0 promoter [209, 210]. Expression of CTCF is regulated by epidermal growth factor (EGF) through activation of NF-κB. CTCF participates in stress-induced signaling cascades, playing a significant antiapoptotic role in cultured human corneal epithelial cells . Oxidative stress activated Bcl3, which in turn suppressed the expression of CTCF through a noncanonical NF-κB pathway [212, 213]. Recent evidence indicates that hypoxic stress induces de-SUMOylation of CTCF to functionally regulate its activity .
9. Actin Cytoskeleton Remodeling and Corneal Gene Expression
Severe corneal abnormalities associated with mutations in actin cytoskeleton remodeling-related proteins such as destrin and KLEIP suggested an essential role for actin in corneal homeostasis [215–222]. Scinderin, an actin depolymerizing protein similar to gelsolin, is abundantly expressed in Zebrafish and Anableps corneas [223–226]. Interestingly, mutations in gelsolin are associated with the Finnish type of familial amyloidosis, a systemic disease characterized by corneal lattice dystrophy, progressive cranial neuropathy, and distal sensorimotor neuropathy in humans [227, 228]. The amyloid subunit in Finnish hereditary amyloidosis is derived from the actin filament-binding region of gelsolin, suggesting the involvement of defective regulation of actin dynamics [229, 230].
The BTB-kelch domain protein KLEIP regulates cell-cell contact formation and cell migration . KLEIP−/− mouse corneas develop normally, but display corneal epithelial hyperplasia and progressive metaplasia leading to total corneal opacity in post-eyelid opening stages . KLEIP−/− corneal stroma was heavily neovascularized and infiltrated with numerous cells . KLEIP−/− corneal epithelium was altered to an epidermal-like structure with superficial keratinized cells and expression of skin markers keratin-1 and loricrin . Molecular mechanism(s) underlying the dramatic phenotype in KLEIP−/− corneas remain(s) to be identified.
Another actin-binding protein implicated in regulating corneal homeostasis is destrin (Dstn), a member of the ADF/cofilin family of proteins that regulates actin dynamics by depolymerizing filamentous actin into monomeric form. A spontaneous mouse mutant in Dstn (corneal disease 1 or Corn1) causes epithelial hyperproliferation and neovascularization . Corn1 mouse cornea is characterized by irregular thickening of the epithelium with increased filamentous actin content, presence of actin stress fibers, and signs of autoinflammatory condition such as increased influx of neutrophils, elevated expression of Cxcl5, and neovascularization [217, 219, 221]. Dstn mutations and resultant changes in actin dynamics have a strong influence on corneal gene expression profile . Microarray analysis revealed dramatic alteration in Corn1 mouse corneal gene expression profile . Genes associated with actin cytoskeleton dynamics were among the most significantly enriched along with a number of serum response factor target genes, suggesting that actin cytoskeleton dynamics regulates SRF-mediated transcriptional control [216, 222]. Consistent with this, conditional ablation of Srf in the Dstn−/− corneal epithelium rescued Corn1 corneal phenotypes including epithelial cell hyperproliferation, inflammation, and neovascularization, confirming an epithelial cell-specific role for SRF .
10. Regulation of Expression of Genes with Important Functions in the Cornea
10.1. Corneal Crystallins
A common assumption in many studies on gene regulation is that gene expression is controlled mainly at the level of transcription. However, post-transcriptional regulation appears to play a significant role in the expression of corneal crystallins aldehyde dehydrogenase IIIA1 (Aldh3a1) and transketolase (Tkt), which constitute roughly 50% and 10% of the water-soluble protein, respectively, and only about 1% each of the total mRNA in the adult cornea . It remains to be determined if this regulation is achieved through increased stability of these specific transcripts and/or their selective overtranslation.
The corneal crystallin Aldh3a1 is expressed at about 500-fold higher level in the mouse corneal epithelial cells than in other tissues . Corneal expression of Aldh3a1 is temporally regulated, increasing by about 100-fold between birth and 6 weeks of age. A 4.4 kb mouse Aldh3a1 promoter fragment was shown to regulate Aldh3a1 expression in the corneal epithelial cells in transgenic mice, suggesting that cis-elements for corneal expression reside within this fragment . The high level of ALDH3 expression involves a strong basal promoter region and a xenobiotic response element (XRE) located within −3.0 kb . ARNT, HNF1, and HNF4 interact with the ALDH3-XRE in an aryl hydrocarbon-receptor-independent, ARNT-requiring manner to influence Aldh3a1 expression . Aldh3a1 promoter activity is controlled synergistically by Pax6, Oct1, p300, KLF4, and KLF5 [66, 232]. A suppressor sequence resides within the first intron of the mouse Aldh3a1 gene, although it is not known how it might influence endogenous gene expression . Unlike the mouse, the rabbit corneas preferentially express aldehyde dehydrogenase class 1 (ALDH1A1) [11, 233]. The −3519 to +43 bp rabbit Aldh1a1 promoter fragment which contains three xenobiotic response elements (XREs) and one E-box element recapitulated this preferential expression in transgenic mice . The hypoxia-inducible factor 3α/aryl hydrocarbon nuclear translocator heterodimer is implicated in Aldh1a1 promoter activation via the XREs .
The second major crystallin in the mouse corneas is transketolase (Tkt) . Tkt mRNA levels increase six-fold in the mouse cornea within 1-2 days of eyelid opening, in a manner dependent on exposure to light and oxidative stress . Two transcription initiation sites separated by 630 bp that shares a common initiator ATG codon have been identified in Tkt gene [234, 235]. The proximal GC-rich transcription initiation site (within intron 1) lacking a TATA box is used for high corneal expression, while the distal transcription initiation site is used weakly in liver. Not much is known about the transcription factors regulating Tkt promoter activity, other than the involvement of Klf4 .
Keratin-12 (Krt12), one of the more than 30 different keratins (intermediate filament components), is abundantly expressed specifically in the stratified corneal epithelium . Heterozygous mutations in KRT12 cause Meesmann’s corneal dystrophy, an autosomal dominant disorder that affects corneal epithelium. Disruption of Krt12 gene results in fragile corneal epithelium resembling Meesmann’s corneal dystrophy in the mouse . From E15.5 to PN10, Krt12 expression is restricted to the suprabasal and/or superficial cells of the corneal epithelium. After PN30, the number of Krt12-positive basal cells increases with age . Particle-mediated gene transfer helped identify corneal epithelial-specific cis-elements within a 2.5 kb Krt12 promoter fragment . Pax6, Klf4 and Klf6 stimulate Krt12 promoter activity [65, 66, 172, 238]. Expression of Krt12 is delayed and downregulated in the Pax6+/− corneal epithelium, implying regulation of Krt12 promoter activity by Pax6 . Interestingly, overexpression of Pax6 also affected Krt12 expression, providing evidence for a tight dosage-dependent influence of Pax6 on Krt12 promoter activity .
Lumican (Lum), a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, and keratocan , is the major keratan sulfate proteoglycan (KSPG) of the cornea. In addition to its high level of expression in the cornea, lumican is also expressed in most interstitial collagenous matrices, including the connective tissues of the heart . Though lumican mRNA is detected early during embryogenesis in the cornea and sclera, the mature sulfated KSPG core proteins can be detected only after the eyes open by PN12 .
Lumican is an important determinant of corneal transparence as shown by the corneal opacity in lumican null mice . Lumican regulates collagen fibril organization, corneal circumferential growth, neutrophil migration in response to bacterial infections, and epithelial cell migration during wound healing and tissue repair [30, 131, 239, 241–247]. Mechanistically, lumican protein binds collagen fibrils allowing the highly charged hydrophilic glycosaminoglycans (GAGs) to regulate interfibrillar spacing, an important determinant of corneal transparence [239, 242, 245]. In spite of its importance, not much is known about the regulation of lumican expression in the cornea.
Keratocan (Kera) is another important KSPG abundantly expressed in the mouse corneal stroma. In humans, mutations in KERA gene are associated with cornea plana (CNA2) characterized by flattened cornea leading to decreased visual acuity. Kera−/− mice exhibit less severe phenotype than the Lum−/− mice [248, 249]. Though Kera−/− corneal stroma was thinner with disorganized and relatively thicker collagen fibrils, its corneal transparence appeared normal, suggesting that the contributions of keratocan towards maintenance of stromal collagenous matrix and corneal transparence are subtle, if any .
The expression of Kera is first detected in the periocular mesenchymal cells at E13.5. After E14.5, Kera expression is restricted to the stromal keratocytes . In adult transgenic mice, expression of β-geo transgene driven by Kera 3.2 kb upstream sequence, exon 1, and 0.4 kb of intron 1, β-Gal activity was detected only in cornea. Spatiotemporal activity of this transgene recapitulated that of endogenous Kera, suggesting that the 3.2 kb upstream sequence contains the necessary cis-elements to regulate keratocan gene expression . Interestingly, lumican and keratocan expressions appear to be coupled, as demonstrated by the increased expression of Kera upon overexpression of lumican and the reduced expression of Kera in Lum−/− mice .
11. Remaining Challenges and Future Prospects
We have witnessed substantial progress in our understanding of gene expression in the ocular surface. We have a good understanding of the expression and function of different transcription factors in regulating gene expression in the ocular surface. However, much of this understanding is restricted to the cornea, with a limited awareness of gene expression in the conjunctiva, lacrimal glands, and meibomian glands. In order to develop novel methods of diagnosis and pharmacotherapeutic intervention in ocular surface disorders, it is necessary to gain complete understanding of the changes in gene expression in the ocular surface and the mechanisms by which they are regulated. In view of the importance of the tear film in ocular surface health, it will be worthwhile studying regulation of gene expression in the lacrimal glands and meibomian glands which contribute the aqueous and lipid layers of the tear film, respectively, in greater detail. Similarly, molecular mechanisms governing the regulation of expression of mucins in the conjunctival goblet cells and corneal epithelial cells are understudied and deserve further exploration .
Four additional areas of research in ocular surface biology stand out as deserving extra attention in the immediate future. First, we have only recently begun to appreciate the depth of the contributions of miRNAs to ocular surface gene regulation and can expect many rapid and exciting new discoveries in this field in the coming years. Second, reliable markers that enable reproducible isolation of corneal limbal epithelial stem cells in a clinical setting have been elusive in spite of the tremendous effort from different laboratories over the last decade, and our effort in this direction needs to be stepped up. Third, changes in expression of the ocular surface and tear film specific markers in response to various proinflammatory chemical, physical, and microbial insults and the specific network of transcription factors responsible for these changes need to be characterized in great detail, facilitating improved diagnosis and therapeutic intervention in ocular surface disorders. Finally, most of the early large-scale ocular surface gene expression studies have focused on the transcript levels. However, multiple studies suggest a significant discordance between transcript and protein levels in a given cell, indicating that regulation at the level of alternate splicing, transcript stability, efficiency of translation, protein stability, and so forth, also play critical roles in gene expression (reviewed in [252, 253]). With the advent of large scale proteomics technologies in the near future, we can expect to uncover if the current findings at the transcript levels truly reflect corresponding protein levels.
Many studies described above have utilized advanced transgenic mouse technologies, germline deletions, and/or conditional inactivation of specific genes in the surface ectoderm-derived tissues of the eye. Further improvements in our ability to delete or mutate specific genes in a precise spatiotemporally regulated manner will further aid this effort. We can look forward with excitement to the identification of additional roles for the transcription factors currently known to influence ocular surface development. In addition, we can expect to discover the contributions of novel transcription factors and their interactions with other factors, revealing the molecular basis of regulation of gene expression in the ocular surface. Finally, ongoing improvements in instrumentation and techniques which facilitate collection of specific subsets of cells from hard-to-reach areas of the eye, coupled with the progress in second generation sequencing-based technologies such as RNA-Seq and ChIP-Seq which facilitate large scale transcriptome analyses at a relatively low cost, are expected to revolutionize our understanding of gene expression in the ocular surface.
The work in the author’s laboratory was supported by NEI K22 EY01687 and NEI 5 P30 EY08098 core Grant, by unrestricted Grants from Research to Prevent Blindness and the Eye and Ear Foundation of Pittsburgh, and by startup funds from the Department of Ophthalmology, University of Pittsburgh. The author thanks Sudha Swamynathan for help in preparation of this paper.
- D. Pascolini and S. P. Mariotti, “Global estimates of visual impairment: 2010,” British Journal of Ophthalmology, vol. 96, pp. 614–618, 2012.
- R. L. Chow and R. A. Lang, “Early eye development in vertebrates,” Annual Review of Cell and Developmental Biology, vol. 17, pp. 255–296, 2001.
- A. Cvekl and E. R. Tamm, “Anterior eye development and ocular mesenchyme: new insights from mouse models and human diseases,” BioEssays, vol. 26, no. 4, pp. 374–386, 2004.
- J. D. Zieske, “Corneal development associated with eyelid opening,” International Journal of Developmental Biology, vol. 48, no. 8-9, pp. 903–911, 2004.
- E. D. Hay, “Development of the vertebrate cornea,” International Review of Cytology, vol. 63, pp. 263–322, 1979.
- J. M. Collinson, L. Morris, A. I. Reid et al., “Clonal analysis of patterns of growth, stem cell activity, and cell movement during the development and maintenance of the murine corneal epithelium,” Developmental Dynamics, vol. 224, no. 4, pp. 432–440, 2002.
- G. Cotsarelis, S. Z. Cheng, G. Dong, T. T. Sun, and R. M. Lavker, “Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells,” Cell, vol. 57, no. 2, pp. 201–209, 1989.
- T. Nagasaki and J. Zha, “Centripetal movement of corneal epithelial cells in the normal adult mouse,” Investigative Ophthalmology and Visual Science, vol. 44, no. 2, pp. 558–566, 2003.
- J. Piatigorsky, “Gene sharing in lens and cornea: facts and implications,” Progress in Retinal and Eye Research, vol. 17, no. 2, pp. 145–174, 1998.
- J. V. Jester, T. Moller-Pedersen, J. Huang et al., “The cellular basis of corneal transparency: evidence for 'corneal crystallins',” Journal of Cell Science, vol. 112, Part 5, pp. 613–622, 1999.
- J. V. Jester, A. Budge, S. Fisher, and J. Huang, “Corneal keratocytes: phenotypic and species differences in abundant protein expression and in vitro light-scattering,” Investigative Ophthalmology and Visual Science, vol. 46, no. 7, pp. 2369–2378, 2005.
- N. Tanifuji-Terai, K. Terai, Y. Hayashi, T. I. Chikama, and W. W. Y. Kao, “Expression of keratin 12 and maturation of corneal epithelium during development and postnatal growth,” Investigative Ophthalmology and Visual Science, vol. 47, no. 2, pp. 545–551, 2006.
- H. P. Makarenkova, M. Ito, V. Govindarajan et al., “FGF10 is an inducer and Pax6 a competence factor for lacrimal gland development,” Development, vol. 127, no. 12, pp. 2563–2572, 2000.
- C. J. Nien, S. Massei, G. Lin et al., “The development of meibomian glands in mice,” Molecular Vision, vol. 16, pp. 1132–1140, 2010.
- V. Govindarajan, M. Ito, H. P. Makarenkova, R. A. Lang, and P. A. Overbeek, “Endogenous and ectopic gland induction by FGF-10,” Developmental Biology, vol. 225, no. 1, pp. 188–200, 2000.
- D. Gupta, S. A. K. Harvey, N. Kaminski, and S. K. Swamynathan, “Mouse conjunctival forniceal gene expression during postnatal development and its regulation by krüppel-like factor 4,” Investigative Ophthalmology and Visual Science, vol. 52, no. 8, pp. 4951–4962, 2011.
- W. Adachi, H. Ulanovsky, Y. Li, B. Norman, J. Davis, and J. Piatigorsky, “Serial analysis of gene expression (SAGE) in the rat limbal and central corneal epithelium,” Investigative Ophthalmology and Visual Science, vol. 47, no. 9, pp. 3801–3810, 2006.
- H. C. Turner, M. T. Budak, M. A. M. Akinci, and J. M. Wolosin, “Comparative analysis of human conjunctival and corneal epithelial gene expression with oligonucleotide microarrays,” Investigative Ophthalmology and Visual Science, vol. 48, no. 5, pp. 2050–2061, 2007.
- M. A. Akinci, H. Turner, M. Taveras et al., “Molecular profiling of conjunctival epithelial side- population stem cells: atypical cell surface markers and sources of a slow-cycling phenotype,” Investigative Ophthalmology and Visual Science, vol. 50, no. 9, pp. 4162–4172, 2009.
- M. A. Akinci, H. Turner, M. Taveras, and J. M. Wolosin, “Differential gene expression in the pig limbal side population: implications for stem cell cycling, replication, and survival,” Investigative Ophthalmology and Visual Science, vol. 50, no. 12, pp. 5630–5638, 2009.
- M. Zhou, X. M. Li, and R. M. Lavker, “Transcriptional profiling of enriched populations of stem cells versus transient amplifying cells: a comparison of limbal and corneal epithelial basal cells,” Journal of Biological Chemistry, vol. 281, no. 28, pp. 19600–19609, 2006.
- A. M. Ozyildirim, G. J. Wistow, J. Gao et al., “The lacrimal gland transcriptome is an unusually rich source of rare and poorly characterized gene transcripts,” Investigative Ophthalmology and Visual Science, vol. 46, no. 5, pp. 1572–1580, 2005.
- J. L. Ubels, H. M. Hoffman, S. Srikanth, J. H. Resau, and C. P. Webb, “Gene expression in rat lacrimal gland duct cells collected using laser capture microdissection: evidence for K+secretion by duct cells,” Investigative Ophthalmology and Visual Science, vol. 47, no. 5, pp. 1876–1885, 2006.
- C. Q. Nguyen, A. Sharma, J. X. She, R. A. McIndoe, and A. B. Peck, “Differential gene expressions in the lacrimal gland during development and onset of keratoconjunctivitis sicca in Sjogren's syndrome (SJS)-like disease of the C57BL/6.NOD-Aec1Aec2 mouse,” Experimental Eye Research, vol. 88, no. 3, pp. 398–409, 2009.
- A. B. Peck, B. T. Saylor, L. Nguyen et al., “Gene expression profiling of early-phase Sjogren's syndrome in C57BL/6.NOD-Aec1Aec2 mice identifies focal adhesion maturation associated with infiltrating leukocytes,” Investigative Ophthalmology & Visual Science, vol. 52, pp. 5647–5655, 2011.
- J. L. Ubels, I. K. Gipson, S. J. Spurr-Michaud, A. S. Tisdale, R. E. Van Dyken, and M. P. Hatton, “Gene expression in human accessory lacrimal glands of wolfring,” Investigative Ophthalmology & Visual Science, vol. 53, no. 11, pp. 6738–6747, 2012.
- S. Liu, S. M. Richards, K. Lo, M. Hatton, A. Fay, and D. A. Sullivan, “Changes in gene expression in human meibomian gland dysfunction,” Investigative Ophthalmology and Visual Science, vol. 52, no. 5, pp. 2727–2740, 2011.
- T. Suzuki, F. Schirra, S. M. Richards, R. V. Jensen, and D. A. Sullivan, “Estrogen and progesterone control of gene expression in the mouse meibomian gland,” Investigative Ophthalmology and Visual Science, vol. 49, no. 5, pp. 1797–1808, 2008.
- D. A. Sullivan, R. V. Jensen, T. Suzuki, and S. M. Richards, “Do sex steroids exert sex-specific and/or opposite effects on gene expression in lacrimal and meibomian glands?” Molecular Vision, vol. 15, pp. 1553–1572, 2009.
- F. Wu, S. Lee, M. Schumacher, A. Jun, and S. Chakravarti, “Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon,” Experimental Eye Research, vol. 87, no. 3, pp. 214–225, 2008.
- B. Norman, J. Davis, and J. Piatigorsky, “Postnatal gene expression in the normal mouse cornea by SAGE,” Investigative Ophthalmology and Visual Science, vol. 45, no. 2, pp. 429–440, 2004.
- D. Kenchegowda, S. A. K. Harvey, S. Swamynathan, K. L. Lathrop, and S. K. Swamynathan, “Critical role of Klf5 in regulating gene expression during post-eyelid opening maturation of mouse corneas,” PLoS One, vol. 7, article e44771, 2012.
- R. E. Hausman, “Ocular extracellular matrices in development,” Progress in Retinal and Eye Research, vol. 26, no. 2, pp. 162–188, 2007.
- J. M. Sivak and M. E. Fini, “MMPs in the eye: emerging roles for matrix metalloproteinases in ocular physiology,” Progress in Retinal and Eye Research, vol. 21, no. 1, pp. 1–14, 2002.
- J. M. Wolosin, M. T. Budak, and M. A. M. Akinci, “Ocular surface epithelial and stem cell development,” International Journal of Developmental Biology, vol. 48, no. 8-9, pp. 981–991, 2004.
- M. E. Fini and B. M. Stramer, “How the cornea heals: cornea-specific repair mechanisms affecting surgical outcomes,” Cornea, vol. 24, no. 8, pp. S2–S11, 2005.
- F. Majo, A. Rochat, M. Nicolas, G. A. Jaoudé, and Y. Barrandon, “Oligopotent stem cells are distributed throughout the mammalian ocular surface,” Nature, vol. 456, no. 7219, pp. 250–254, 2008.
- S. Burman and V. Sangwan, “Cultivated limbal stem cell transplantation for ocular surface reconstruction,” Journal of Clinical Ophthalmology, vol. 2, pp. 489–502, 2008.
- M. Fernandes, V. S. Sangwan, S. K. Rao et al., “Limbal Stem Cell Transplantation,” Indian Journal of Ophthalmology, vol. 52, no. 1, pp. 5–22, 2004.
- V. S. Sangwan, S. Basu, S. MacNeil, and D. Balasubramanian, “Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency,” British Journal of Ophthalmology, vol. 96, pp. 931–934, 2012.
- V. S. Sangwan, G. K. Vemuganti, S. Singh, and D. Balasubramanian, “Successful reconstruction of damaged ocular outer surface in humans using limbal and conjuctival stem cell culture methods,” Bioscience Reports, vol. 23, no. 4, pp. 169–174, 2003.
- G. K. Vemuganti, V. S. Sangwan, and G. N. Rao, “The promise of stem cell therapy for eye disorders: guest Editorial,” Clinical and Experimental Optometry, vol. 90, no. 5, pp. 315–316, 2007.
- P. Rama, S. Matuska, G. Paganoni, A. Spinelli, M. De Luca, and G. Pellegrini, “Limbal stem-cell therapy and long-term corneal regeneration,” The New England Journal of Medicine, vol. 363, no. 2, pp. 147–155, 2010.
- B. B. Kulkarni, P. J. Tighe, I. Mohammed et al., “Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics,” BMC Genomics, vol. 11, no. 1, article 526, 2010.
- V. Barbaro, A. Testa, E. Di Iorio, F. Mavilio, G. Pellegrini, and M. De Luca, “C/EBPδ regulates cell cycle and self-renewal of human limbal stem cells,” Journal of Cell Biology, vol. 177, no. 6, pp. 1037–1049, 2007.
- E. C. Figueira, N. Di Girolamo, M. T. Coroneo, and D. Wakefield, “The phenotype of limbal epithelial stem cells,” Investigative Ophthalmology and Visual Science, vol. 48, no. 1, pp. 144–156, 2007.
- R. Hayashi, M. Yamato, H. Sugiyama et al., “N-cadherin is expressed by putative stem/progenitor cells and melanocytes in the human limbal epithelial stem cell niche,” Stem Cells, vol. 25, no. 2, pp. 289–296, 2007.
- H. Qi, D. Q. Li, H. D. Shine et al., “Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells,” Experimental Eye Research, vol. 86, no. 1, pp. 34–40, 2008.
- P. B. Thomas, Y. H. Liu, F. F. Zhuang et al., “Identification of Notch-1 expression in the limbal basal epithelium,” Molecular Vision, vol. 13, pp. 337–344, 2007.
- L. Takács, E. Tóth, G. Losonczy et al., “Differentially expressed genes associated with human limbal epithelial phenotypes: new molecules that potentially facilitate selection of stem cell-enriched populations,” Investigative Ophthalmology and Visual Science, vol. 52, no. 3, pp. 1252–1260, 2011.
- M. Lyngholm, P. E. Høyer, H. Vorum, K. Nielsen, N. Ehlers, and K. Møllgård, “Immunohistochemical markers for corneal stem cells in the early developing human eye,” Experimental Eye Research, vol. 87, no. 2, pp. 115–121, 2008.
- M. Lyngholm, H. Vorum, K. Nielsen, M. Ostergaard, B. Honoré, and N. Ehlers, “Differences in the protein expression in limbal versus central human corneal epithelium—a search for stem cell markers,” Experimental Eye Research, vol. 87, no. 2, pp. 96–105, 2008.
- M. N. Nakatsu, Z. Ding, M. Y. Ng, T. T. Truong, F. Yu, and S. X. Deng, “Wnt/beta-catenin signaling regulates proliferation of human cornea epithelial stem/progenitor cells,” Investigative Ophthalmology & Visual Science, vol. 52, pp. 4734–4741, 2011.
- Y. J. Hsueh, P. C. Kuo, and J. K. Chen, “Transcriptional regulators of the DeltaNp63: their role in limbal epithelial cell proliferation,” Journal of Cellular Physiologyl, vol. 228, no. 3, pp. 536–546, 2013.
- R. Lu, Y. Qu, J. Ge et al., “Transcription factor TCF4 maintains the properties of human corneal epithelial stem cells,” Stem Cells, vol. 30, pp. 753–761, 2012.
- R. A. Thoft, “The role of the limbus in ocular surface maintenance and repair,” Acta Ophthalmologica, vol. 67, no. 192, pp. 91–94, 1989.
- R. A. Thoft, L. A. Wiley, and N. Sundarraj, “The multipotential cells of the limbus,” Eye, vol. 3, Part 2, pp. 109–113, 1989.
- I. K. Gipson, “The ocular surface: the challenge to enable and protect vision. The Friedenwald lecture,” Investigative Ophthalmology & Visual Science, vol. 48, no. 10, pp. 4391–4398, 2007.
- H. Wan, K. H. Kaestner, S. L. Ang et al., “Foxa2 regulates alveolarization and goblet cell hyperplasia,” Development, vol. 131, no. 4, pp. 953–964, 2004.
- D. Z. Ye and K. H. Kaestner, “Foxa1 and Foxa2 Control the Differentiation of Goblet and Enteroendocrine L- and D-Cells in Mice,” Gastroenterology, vol. 137, no. 6, pp. 2052–2062, 2009.
- K. S. Park, T. R. Korfhagen, M. D. Bruno et al., “SPDEF regulates goblet cell hyperplasia in the airway epithelium,” Journal of Clinical Investigation, vol. 117, no. 4, pp. 978–988, 2007.
- G. Chen, T. R. Korfhagen, Y. Xu et al., “SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production,” Journal of Clinical Investigation, vol. 119, no. 10, pp. 2914–2924, 2009.
- A. Gregorieff, D. E. Stange, P. Kujala et al., “The ets-domain transcription factor spdef promotes maturation of goblet and paneth cells in the intestinal epithelium,” Gastroenterology, vol. 137, no. 4, pp. 1333–1345, 2009.
- D. Kenchegowda, S. Swamynathan, D. Gupta, H. Wan, J. Whitsett a, and S. K. Swamynathan, “Conditional disruption of mouse Klf5 results in defective eyelids with malformed meibomian glands, abnormal cornea and loss of conjunctival goblet cells,” Developmental Biology, vol. 356, no. 1, pp. 5–18, 2011.
- S. K. Swamynathan, J. P. Katz, K. H. Kaestner, R. Ashery-Padan, M. A. Crawford, and J. Piatigorsky, “Conditional deletion of the mouse Klf4 gene results in corneal epithelial fragility, stromal edema, and loss of conjunctival goblet cells,” Molecular and Cellular Biology, vol. 27, no. 1, pp. 182–194, 2007.
- S. K. Swamynathan, J. Davis, and J. Piatigorsky, “Identification of candidate Klf4 target genes reveals the molecular basis of the diverse regulatory roles of Klf4 in the mouse cornea,” Investigative Ophthalmology and Visual Science, vol. 49, no. 8, pp. 3360–3370, 2008.
- T. K. Noah, A. Kazanjian, J. Whitsett, and N. F. Shroyer, “SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells,” Experimental Cell Research, vol. 316, no. 3, pp. 452–465, 2010.
- W. P. Kloosterman and R. H. A. Plasterk, “The diverse functions of microRNAs in animal development and disease,” Developmental Cell, vol. 11, no. 4, pp. 441–450, 2006.
- D. G. Ryan, M. Oliveira-Fernandes, and R. M. Lavker, “MicroRNAs of the mammalian eye display distinct and overlapping tissue specificity,” Molecular Vision, vol. 12, pp. 1175–1184, 2006.
- M. Karali, I. Peluso, V. A. Gennarino et al., “MiRNeye: a microRNA expression atlas of the mouse eye,” BMC Genomics, vol. 11, no. 1, article 715, 2010.
- M. Karali, I. Peluso, V. Marigo, and S. Banfi, “Identification and characterization of micrornas expressed in the mouse eye,” Investigative Ophthalmology & Visual Science, vol. 48, no. 2, pp. 509–515, 2007.
- Y. Li and J. Piatigorsky, “Targeted deletion of dicer disrupts lens morphogenesis, corneal epithelium stratification, and whole eye development,” Developmental Dynamics, vol. 238, no. 9, pp. 2388–2400, 2009.
- A. E. Hughes, D. T. Bradley, M. Campbell et al., “Mutation altering the miR-184 seed region causes familial keratoconus with cataract,” The American Journal of Human Genetics, vol. 89, pp. 628–633, 2011.
- R. Shalom-Feuerstein, L. Serror, S. F. Divonne et al., “Pluripotent stem cell model reveals essential roles for miR-450b-5p and miR-184 in embryonic corneal lineage specification,” Stem Cells, vol. 30, pp. 898–909, 2012.
- J. Yu, D. G. Ryan, S. Getsios, M. Oliveira-Fernandes, A. Fatima, and R. M. Lavker, “MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 49, pp. 19300–19305, 2008.
- S. K. W. Lee, Y. Teng, H. K. Wong et al., “MicroRNA-145 regulates human corneal epithelial differentiation,” PLoS ONE, vol. 6, no. 6, article e21249, Article ID e21249, 2011.
- W. J. Gehring, “Historical perspective on the development and evolution of eyes and photoreceptors,” International Journal of Developmental Biology, vol. 48, no. 8-9, pp. 707–717, 2004.
- X. Zhang, A. Friedman, S. Heaney, P. Purcell, and R. L. Maas, “Meis homeoproteins directly regulate Pax6 during vertebrate lens morphogenesis,” Genes and Development, vol. 16, no. 16, pp. 2097–2107, 2002.
- R. Ashery-Padan, T. Marquardt, X. Zhou, and P. Gruss, “Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye,” Genes and Development, vol. 14, no. 21, pp. 2701–2711, 2000.
- B. M. Koroma, J. M. Yang, and O. H. Sundin, “The Pax-6 homeobox gene is expressed throughout the corneal and conjunctival epithelia,” Investigative Ophthalmology & Visual Science, vol. 38, no. 1, pp. 108–120, 1997.
- T. I. Simpson and D. J. Price, “Pax6; a pleiotropic player in development,” BioEssays, vol. 24, no. 11, pp. 1041–1051, 2002.
- J. M. Collinson, J. C. Quinn, R. E. Hill, and J. D. West, “The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo,” Developmental Biology, vol. 255, no. 2, pp. 303–312, 2003.
- J. Davis, M. K. Duncan, W. G. Robison Jr., and J. Piatigorsky, “Requirement for Pax6 in corneal morphogenesis: a role in adhesion,” Journal of Cell Science, vol. 116, no. 11, pp. 2157–2167, 2003.
- L. J. Leiper, J. Ou, P. Walczysko et al., “Control of patterns of corneal innervation by Pax6,” Investigative Ophthalmology & Visual Science, vol. 50, pp. 1122–1128, 2009.
- J. Graw, “The genetic and molecular basis of congenital eye defects,” Nature Reviews Genetics, vol. 4, no. 11, pp. 876–888, 2003.
- I. M. Hanson, J. M. Fletcher, T. Jordan et al., “Mutations at the PAX6 locus are found in heterogeneous anterior segment malformations including Peter's anomaly,” Nature Genetics, vol. 6, no. 2, pp. 168–173, 1994.
- T. Jordan, I. Hanson, D. Zaletayev et al., “The human PAX6 gene is mutated in two patients with aniridia,” Nature Genetics, vol. 1, no. 5, pp. 328–332, 1992.
- I. M. Hanson, “PAX6 and Congenital Eye Malformations,” Pediatric Research, vol. 54, no. 6, pp. 791–796, 2003.
- B. L. M. Hogan, E. M. A. Hirst, G. Horsburgh, and C. M. Hetherington, “Small eye (Sey): a mouse model for the genetic analysis of craniofacial abnormalities,” Development, vol. 103, pp. 115–119, 1988.
- T. Ramaesh, J. M. Collinson, K. Ramaesh, M. H. Kaufman, J. D. West, and B. Dhillon, “Corneal abnormalities in Pax6+/- small eye mice mimic human aniridia-related keratopathy,” Investigative Ophthalmology and Visual Science, vol. 44, no. 5, pp. 1871–1878, 2003.
- J. M. Collinson, J. C. Quinn, M. A. Buchanan et al., “Primary defects in the lens underlie complex anterior segment abnormalities of the Pax6 heterozygous eye,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 17, pp. 9688–9693, 2001.
- R. Kucerova, J. Ou, D. Lawson, L. J. Leiper, and J. M. Collinson, “Cell surface glycoconjugate abnormalities and corneal epithelial wound healing in the Pax6+/- mouse model of aniridia-related keratopathy,” Investigative Ophthalmology & Visual Science, vol. 47, no. 12, pp. 5276–5282, 2006.
- S. Kanakubo, T. Nomura, K. I. Yamamura, J. I. Miyazaki, M. Tamai, and N. Osumi, “Abnormal migration and distribution of neural crest cells in Pax6 heterozygous mutant eye, a model for human eye diseases,” Genes to Cells, vol. 11, no. 8, pp. 919–933, 2006.
- N. Dorà, J. Ou, R. Kucerova, I. Parisi, J. D. West, and J. M. Collinson, “PAX6 dosage effects on corneal development, growth, and wound healing,” Developmental Dynamics, vol. 237, no. 5, pp. 1295–1306, 2008.
- J. Davis and J. Piatigorsky, “Overexpression of Pax6 in mouse cornea directly alters corneal epithelial cells: changes in immune functionvascularization, and differentiation,” Investigative Ophthalmology & Visual Science, vol. 52, pp. 4158–4168, 2011.
- J. Ouyang, Y. C. Shen, L. K. Yeh et al., “Pax6 overexpression suppresses cell proliferation and retards the cell cycle in corneal epithelial cells,” Investigative Ophthalmology & Visual Science, vol. 47, no. 6, pp. 2397–2407, 2006.
- R. Kucerova, N. Dora, R. L. Mort et al., “Interaction between hedgehog signalling and PAX6 dosage mediates maintenance and regeneration of the corneal epithelium,” Molecular Vision, vol. 18, pp. 139–150, 2012.
- R. L. Mort, A. J. Bentley, F. L. Martin et al., “Effects of aberrant Pax6 gene dosage on mouse corneal pathophysiology and corneal epithelial homeostasis,” PLoS One, vol. 6, article e28895, 2011.
- S. I. Aota, N. Nakajima, R. Sakamoto, S. Watanabe, N. Ibaraki, and K. Okazaki, “Pax6 autoregulation mediated by direct interaction of Pax6 protein with the head surface ectoderm-specific enhancer of the mouse Pax6 gene,” Developmental Biology, vol. 257, no. 1, pp. 1–13, 2003.
- I. Brownell, M. Dirksen, and M. Jamrich, “Forkhead Foxe3 maps to the dysgenetic lens locus and is critical in lens development and differentiation,” Genesis, vol. 27, pp. 81–93, 2000.
- B. K. Chauhan, N. A. Reed, Y. Yang et al., “A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens,” Genes to Cells, vol. 7, no. 12, pp. 1267–1283, 2002.
- A. Cvekl, Y. Yang, B. K. Chauhan, and K. Cveklova, “Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens,” International Journal of Developmental Biology, vol. 48, no. 8-9, pp. 829–844, 2004.
- M. K. Duncan, A. Cvekl, X. Li, and J. Piatigorsky, “Truncated forms of Pax-6 disrupt lens morphology in transgenic mice,” Investigative Ophthalmology & Visual Science, vol. 41, no. 2, pp. 464–473, 2000.
- Y. Furuta and B. L. M. Hogan, “BMP4 is essential for lens induction in the mouse embryo,” Genes and Development, vol. 12, no. 23, pp. 3764–3775, 1998.
- T. Marquardt, R. Ashery-Padan, N. Andrejewski, R. Scardigli, F. Guillemot, and P. Gruss, “Pax6 is required for the multipotent state of retinal progenitor cells,” Cell, vol. 105, no. 1, pp. 43–55, 2001.
- P. A. Zaki, J. M. Collinson, J. Toraiwa, T. I. Simpson, D. J. Price, and J. C. Quinn, “Penetrance of eye defects in mice heterozygous for mutation of Gli3 is enhanced by heterozygous mutation of Pax6,” BMC Developmental Biology, vol. 6, article 46, 2006.
- W. Liu, O. V. Lagutin, M. Mende, A. Streit, and G. Oliver, “Six3 activation of Pax6 expression is essential for mammalian lens induction and specification,” EMBO Journal, vol. 25, no. 22, pp. 5383–5395, 2006.
- O. Lagutin, C. C. Zhu, Y. Furuta, D. H. Rowitch, A. P. McMahon, and G. Oliver, “Six3 promotes the formation of ectopic optic vesicle-like structures in mouse embryos,” Developmental Dynamics, vol. 221, no. 3, pp. 342–349, 2001.
- G. Oliver, A. Mailhos, R. Wehr, N. G. Copeland, N. A. Jenkins, and P. Gruss, “Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development,” Development, vol. 121, no. 12, pp. 4045–4055, 1995.
- G. Goudreau, P. Petrou, L. W. Reneker, J. Graw, J. Löster, and P. Gruss, “Mutually regulated expression of Pax6 and Six3 and its implications for the Pax6 haploinsufficient lens phenotype,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 13, pp. 8719–8724, 2002.
- G. A. Secker and J. T. Daniels, “Corneal epithelial stem cells: deficiency and regulation,” Stem Cell Reviews, vol. 4, no. 3, pp. 159–168, 2008.
- W. Li, Y. T. Chen, Y. Hayashida et al., “Down-regulation of Pax6 is associated with abnormal differentiation of corneal epithelial cells in severe ocular surface diseases,” Journal of Pathology, vol. 214, no. 1, pp. 114–122, 2008.
- T. Ramaesh, K. Ramaesh, J. M. Collinson, S. A. Chanas, B. Dhillon, and J. D. West, “Developmental and cellular factors underlying corneal epithelial dysgenesis in the Pax6+/- mouse model of aniridia,” Experimental Eye Research, vol. 81, no. 2, pp. 224–235, 2005.
- J. M. Sivak, J. A. West-Mays, A. Yee, T. Williams, and M. E. Fini, “Transcription factors Pax6 and AP-2α interact to coordinate corneal epithelial repair by controlling expression of matrix metalloproteinase gelatinase B,” Molecular and Cellular Biology, vol. 24, no. 1, pp. 245–257, 2004.
- J. M. Sivak, R. Mohan, W. B. Rinehart, P. X. Xu, R. L. Maas, and M. E. Fini, “Pax-6 expression and activity are induced in the reepithelializing cornea and control activity of the transcriptional promoter for matrix metalloproteinase gelatinase B,” Developmental Biology, vol. 222, no. 1, pp. 41–54, 2000.
- A. L. Evans and P. J. Gage, “Expression of the homeobox gene Pitx2 in neural crest is required for optic stalk and ocular anterior segment development,” Human Molecular Genetics, vol. 14, no. 22, pp. 3347–3359, 2005.
- M. Asai-Coakwell, C. Backhouse, R. J. Casey, P. J. Gage, and O. J. Lehmann, “Reduced human and murine corneal thickness in an Axenfeld-Rieger syndrome subtype,” Investigative Ophthalmology & Visual Science, vol. 47, no. 11, pp. 4905–4909, 2006.
- J. Holmberg, C. Y. Liu, and T. A. Hjalt, “PITX2 gain-of-function in Rieger syndrome eye model,” American Journal of Pathology, vol. 165, no. 5, pp. 1633–1641, 2004.
- P. J. Gage, M. Qian, D. Wu, and K. I. Rosenberg, “The canonical Wnt signaling antagonist DKK2 is an essential effector of PITX2 function during normal eye development,” Developmental Biology, vol. 317, no. 1, pp. 310–324, 2008.
- S. Kumar and G. Duester, “Retinoic acid signaling in perioptic mesenchyme represses Wnt signaling via induction of Pitx2 and Dkk2,” Developmental Biology, vol. 340, no. 1, pp. 67–74, 2010.
- J. Weng, J. Luo, X. Cheng et al., “Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 16, pp. 6081–6086, 2008.
- M. M. Lucey, Y. Wang, M. Bustin, and M. K. Duncan, “Differential expression of the HMGN family of chromatin proteins during ocular development,” Gene Expression Patterns, vol. 8, no. 6, pp. 433–437, 2008.
- Y. Birger, J. Davis, T. Furusawa, E. Rand, J. Piatigorsky, and M. Bustin, “A role for chromosomal protein HMGN1 in corneal maturation,” Differentiation, vol. 74, no. 1, pp. 19–29, 2006.
- D. Weigel and H. Jackle, “The fork head domain: a novel DNA binding motif of eukaryotic transcription factors?” Cell, vol. 63, no. 3, pp. 455–456, 1990.
- G. Tuteja and K. H. Kaestner, “SnapShot: forkhead transcription factors II,” Cell, vol. 131, no. 1, p. 192, 2007.
- G. Tuteja and K. H. Kaestner, “SnapShot: forkhead transcription factors I,” Cell, vol. 130, no. 6, p. 1160, 2007.
- K. H. Kaestner, W. Knöchel, and D. E. Martínez, “Unified nomenclature for the winged helix/forkhead transcription factors,” Genes and Development, vol. 14, no. 2, pp. 142–146, 2000.
- O. J. Lehmann, J. C. Sowden, P. Carlsson, T. Jordan, and S. S. Bhattacharya, “Fox's in development and disease,” Trends in Genetics, vol. 19, no. 6, pp. 339–344, 2003.
- A. Blixt, H. Landgren, B. R. Johansson, and P. Carlsson, “Foxe3 is required for morphogenesis and differentiation of the anterior segment of the eye and is sensitive to Pax6 gene dosage,” Developmental Biology, vol. 302, no. 1, pp. 218–229, 2007.
- H. Hiemisch, G. Schütz, and K. H. Kaestner, “The mouse Fkh1/Mf1 gene: cDNA sequence, chromosomal localization and expression in adult tissues,” Gene, vol. 220, no. 1-2, pp. 77–82, 1998.
- H. K. Hong, J. H. Lass, and A. Chakravarti, “Pleiotropic skeletal and ocular phenotypes of the mouse mutation congenital hydrocephalus (ch/Mf1) arise from a winged helix/forkhead transcription factor gene,” Human Molecular Genetics, vol. 8, no. 4, pp. 625–637, 1999.
- R. A. Honkanen, D. Y. Nishimura, R. E. Swiderski et al., “A family with Axenfeld-Rieger syndrome and Peters Anomaly caused by a point mutation (Phe112Ser) in the FOXC1 gene,” American Journal of Ophthalmology, vol. 135, no. 3, pp. 368–375, 2003.
- S. H. Kidson, T. Kume, K. Deng, V. Winfrey, and B. L. M. Hogan, “The forkhead/winged-helix gene, Mf1, is necessary for the normal development of the cornea and formation of the anterior chamber in the mouse eye,” Developmental Biology, vol. 211, no. 2, pp. 306–322, 1999.
- O. Lehmann, S. Tuft, G. Brice et al., “Novel anterior segment phenotypes resulting from forkhead gene alterations: evidence for cross-species conservation of function,” Investigative Ophthalmology & Visual Science, vol. 44, no. 6, pp. 2627–2633, 2003.
- D. Mattiske, P. Sommer, S. H. Kidson, and B. L. M. Hogan, “The role of the forkhead transcription factor, Foxc1, in the development of the mouse lacrimal gland,” Developmental Dynamics, vol. 235, no. 4, pp. 1074–1080, 2006.
- R. McKeone, H. Vieira, K. Gregory-Evans, C. Y. Gregory-Evans, and P. Denny, “Foxf2: a novel locus for anterior segment dysgenesis adjacent to the Foxc1 gene,” PLoS One, vol. 6, article e25489, 2011.
- M. Ormestad, A. Blixt, A. Churchill, T. Martinsson, S. Enerbäck, and P. Carlsson, “Foxe3 haploinsufficiency in mice: a model for Peters' anomaly,” Investigative Ophthalmology & Visual Science, vol. 43, no. 5, pp. 1350–1357, 2002.
- S. Seo, H. P. Singh, P. M. Lacal et al., “Forkhead box transcription factor FoxC1 preserves corneal transparency by regulating vascular growth,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, pp. 2015–2020, 2012.
- R. S. Smith, A. Zabaleta, T. Kume et al., “Haploinsufficiency of the transcription factors FOXC1 and FOXC2 results in aberrant ocular development,” Human Molecular Genetics, vol. 9, no. 7, pp. 1021–1032, 2000.
- J. Zhao, K. Kawai, H. Wang et al., “Loss of Msx2 function down-regulates the FoxE3 expression and results in anterior segment dysgenesis resembling Peters anomaly,” American Journal of Pathology, vol. 180, pp. 2230–2239, 2012.
- T. Kume, K. Y. Deng, V. Winfrey, D. B. Gould, M. A. Walter, and B. L. M. Hogan, “The forkhead/winged helix gene Mf1 is disrupted in the pleiotropic mouse mutation congenital hydrocephalus,” Cell, vol. 93, no. 6, pp. 985–996, 1998.
- A. J. Mears, T. Jordan, F. Mirzayans et al., “Mutations of the forkhead/winged-helix gene, FKHL7, in patients with Axenfeld-Rieger anomaly,” American Journal of Human Genetics, vol. 63, no. 5, pp. 1316–1328, 1998.
- F. Mirzayans, D. B. Gould, E. Héon et al., “Axenfeld-Rieger syndrome resulting from mutation of the FKHL7 gene on chromosome 6p25,” European Journal of Human Genetics, vol. 8, no. 1, pp. 71–74, 2000.
- D. Y. Nishimura, R. E. Swiderski, W. L. M. Alward et al., “The forkhead transcription factor gene FKHL7 is responsible for glaucoma phenotypes which map to 6p25,” Nature Genetics, vol. 19, no. 2, pp. 140–147, 1998.
- T. A. Hjalt and E. V. Semina, “Current molecular understanding of Axenfeld-Rieger syndrome,” Expert Reviews in Molecular Medicine, vol. 7, no. 25, pp. 1–17, 2005.
- B. K. Ambati, M. Nozaki, N. Singh et al., “Corneal avascularity is due to soluble VEGF receptor-1,” Nature, vol. 443, no. 7114, pp. 993–997, 2006.
- C. Cursiefen, L. Chen, M. Saint-Geniez et al., “Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 30, pp. 11405–11410, 2006.
- S. Chakrabarti, M. Ramappa, S. Chaurasia, I. Kaur, and A. K. Mandal, “FOXC1-associated phenotypes in humans may not always exhibit corneal neovascularization,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. E1509, 2012.
- J. C. Sowden, “Molecular and developmental mechanisms of anterior segment dysgenesis,” Eye, vol. 21, no. 10, pp. 1310–1318, 2007.
- L. M. Reis and E. V. Semina, “Genetics of anterior segment dysgenesis disorders,” Current Opinion in Ophthalmology, vol. 22, pp. 314–324, 2011.
- A. Blixt, M. Mahlapuu, M. Aitola, M. Pelto-Huikko, S. Enerbäck, and P. Carlsson, “A forkhead gene, FoxE3, is essential for lens epithelial proliferation and closure of the lens vesicle,” Genes and Development, vol. 14, no. 2, pp. 245–254, 2000.
- S. K. Swamynathan, “Krüppel-like factors: three fingers in control,” Human genomics, vol. 4, no. 4, pp. 263–270, 2010.
- F. Chiambaretta, F. De Graeve, G. Turet et al., “Cell and tissue specific expression of human Krüppel-like transcription factors in human ocular surface,” Molecular Vision, vol. 10, pp. 901–909, 2004.
- H. Nakamura, J. Ueda, J. Sugar, and B. Y. J. T. Yue, “Developmentally regulated expression of Sp1 in the mouse cornea,” Investigative Ophthalmology & Visual Science, vol. 46, no. 11, pp. 4092–4096, 2005.
- R. B. Whitelock, Y. Li, L. Zhou, J. Sugar, and B. Y. J. T. Yue, “Expression of transcription factors in keratoconus, a cornea-thinning disease,” Biochemical and Biophysical Research Communications, vol. 235, no. 1, pp. 253–258, 1997.
- X. Shen, J. S. Park, Y. Qiu, J. Sugar, and B. Y. J. T. Yue, “Effects of Sp1 overexpression on cultured human corneal stromal cells,” Genes to Cells, vol. 14, no. 10, pp. 1133–1139, 2009.
- Y. Li, L. Zhou, S. S. Twining, J. Sugar, and B. Y. J. T. Yue, “Involvement of Sp1 elements in the promoter activity of the α1- proteinase inhibitor gene,” Journal of Biological Chemistry, vol. 273, no. 16, pp. 9959–9965, 1998.
- Y. Maruyama, X. Wang, Y. Li, J. Sugar, and B. Y. J. T. Yue, “Involvement of Sp1 elements in the promoter activity of genes affected in keratoconus,” Investigative Ophthalmology & Visual Science, vol. 42, no. 9, pp. 1980–1985, 2001.
- T. T. Chen, R. L. Wu, F. Castro-Munozledo, and T. T. Sun, “Regulation of K3 keratin gene transcription by Sp1 and AP-2 in differentiating rabbit corneal epithelial cells,” Molecular and Cellular Biology, vol. 17, no. 6, pp. 3056–3064, 1997.
- R. L. Wu, T. T. Chen, and T. T. Sun, “Functional importance of an Sp1- and an NFkB-related nuclear protein in a keratinocyte-specific promoter of rabbit K3 keratin gene,” Journal of Biological Chemistry, vol. 269, no. 45, pp. 28450–28459, 1994.
- O. G. Opitz and A. K. Rustgi, “Interaction between Sp1 and cell cycle regulatory proteins is important in transactivation of a differentiation-related gene,” Cancer Research, vol. 60, no. 11, pp. 2825–2830, 2000.
- G. Adhikary, J. F. Crish, R. Gopalakrishnan, F. Bone, and R. L. Eckert, “Involucrin expression in the corneal epithelium: an essential role for Sp1 transcription factors,” Investigative Ophthalmology & Visual Science, vol. 46, no. 9, pp. 3109–3120, 2005.
- M. È. Gingras, B. Masson-Gadais, K. Zaniolo et al., “Differential binding of the transcription factors Sp1, AP-1, and NFI to the promoter of the human α5 integrin gene dictates its transcriptional activity,” Investigative Ophthalmology & Visual Science, vol. 50, no. 1, pp. 57–67, 2009.
- R. Mohan, W. B. Rinehart, P. Bargagna-Mohan, and M. E. Fini, “Gelatinase B/lacZ transgenic mice, a model for mapping gelatinase B expression during developmental and injury-related tissue remodeling,” Journal of Biological Chemistry, vol. 273, no. 40, pp. 25903–25914, 1998.
- K. Takahashi and S. Yamanaka, “Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors,” Cell, vol. 126, no. 4, pp. 663–676, 2006.
- J. P. Katz, N. Perreault, B. G. Goldstein et al., “The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon,” Development, vol. 129, no. 11, pp. 2619–2628, 2002.
- R. D. Young, S. K. Swamynathan, C. Boote et al., “Stromal edema in Klf4 conditional null mouse cornea is associated with altered collagen fibril organization and reduced proteoglycans,” Investigative Ophthalmology & Visual Science, vol. 50, no. 9, pp. 4155–4161, 2009.
- J. A. Segre, C. Bauer, and E. Fuchs, “Klf4 is a transcription factor required for establishing the barrier function of the skin,” Nature Genetics, vol. 22, no. 4, pp. 356–360, 1999.
- S. Swamynathan, D. Kenchegowda, J. Piatigorsky, and S. Swamynathan, “Regulation of corneal epithelial barrier function by Krüppel-like transcription factor 4,” Investigative Ophthalmology & Visual Science, vol. 52, no. 3, pp. 1762–1769, 2011.
- H. Wan, F. Luo, S. E. Wert et al., “Kruppel-like factor 5 is required for perinatal lung morphogenesis and function,” Development, vol. 135, no. 15, pp. 2563–2572, 2008.
- H. Nakamura, F. Chiambaretta, J. Sugar, V. Sapin, and B. Y. J. T. Yue, “Developmentally regulated expression of KLF6 in the mouse cornea and lens,” Investigative Ophthalmology & Visual Science, vol. 45, no. 12, pp. 4327–4332, 2004.
- F. Chiambaretta, L. Blanchon, B. Rabier et al., “Regulation of corneal keratin-12 gene expression by the human Krüppel-like transcription factor 6,” Investigative Ophthalmology & Visual Science, vol. 43, no. 11, pp. 3422–3429, 2002.
- F. Chiambaretta, H. Nakamura, F. De Graeve et al., “Krüppel-like factor 6 (KLF6) affects the promoter activity of the α1-proteinase inhibitor gene,” Investigative Ophthalmology & Visual Science, vol. 47, no. 2, pp. 582–590, 2006.
- J. G. Ilagan, A. Cvekl, M. Kantorow, J. Piatigorsky, and C. M. Sax, “Regulation of αA-crystallin gene expression: lens specificity achieved through the differential placement of similar transcriptional control elements in mouse and chicken,” Journal of Biological Chemistry, vol. 274, no. 28, pp. 19973–19978, 1999.
- K. Shirai, S. Saika, Y. Okada, E. Senba, and Y. Ohnishi, “Expression of c-Fos and c-Jun in developing mouse lens,” Ophthalmic Research, vol. 36, no. 4, pp. 226–230, 2004.
- Y. Okada, S. Saika, K. Shirai, Y. Ohnishi, and E. Senba, “Expression of AP-1 (c-Fos/c-Jun) in developing mouse corneal epithelium,” Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 241, no. 4, pp. 330–333, 2003.
- G. Adhikary, J. F. Crish, F. Bone, R. Gopalakrishnan, J. Lass, and R. L. Eckert, “An involucrin promoter AP1 transcription factor binding site is required for expression of involucrin in the corneal epithelium in vivo,” Investigative Ophthalmology & Visual Science, vol. 46, no. 4, pp. 1219–1227, 2005.
- D. Eckert, S. Buhl, S. Weber, R. Jäger, and H. Schorle, “The AP-2 family of transcription factors,” Genome Biology, vol. 6, no. 13, article 246, 2005.
- J. A. West-Mays, J. M. Sivak, S. S. Papagiotas et al., “Positive influence of AP-2α transcription factor on cadherin gene expression and differentiation of the ocular surface,” Differentiation, vol. 71, no. 3, pp. 206–216, 2003.
- C. Ohtaka-Maruyama, F. Hanaoka, and A. B. Chepelinsky, “A novel alternative spliced variant of the transcription factor AP2α is expressed in the murine ocular lens,” Developmental Biology, vol. 202, no. 1, pp. 125–135, 1998.
- J. A. West-Mays, J. Zhang, T. Nottoli et al., “AP-2α transcription factor is required for early morphogenesis of the lens vesicle,” Developmental Biology, vol. 206, no. 1, pp. 46–62, 1999.
- D. J. Dwivedi, G. F. Pontoriero, R. Ashery-Padan, S. Sullivan, T. Williams, and J. A. West-Mays, “Targeted deletion of AP-2α leads to disruption in corneal epithelial cell integrity and defects in the corneal stroma,” Investigative Ophthalmology & Visual Science, vol. 46, no. 10, pp. 3623–3630, 2005.
- W. Naib-Majani, W. Breipohl, E. E. Shazli et al., “The Ets-1 transcription factor is involved in pterygial angiogenesis,” Journal of Veterinary Medicine C, vol. 36, no. 2, pp. 107–110, 2007.
- N. Yoshida, S. Yoshida, M. Araie, H. Handa, and Y. I. Nabeshima, “Ets family transcription factor ESE-1 expressed in corneal epithelial cells and is involved in their differentiation,” Mechanisms of Development, vol. 97, no. 1-2, pp. 27–34, 2000.
- T. J. Liesegang, “Physiologic changes of the cornea with contact lens wear,” CLAO Journal, vol. 28, no. 1, pp. 12–27, 2002.
- R. B. Mandell and I. Fatt, “Thinning of the human cornea on awakening ,” Nature, vol. 208, no. 5007, pp. 292–293, 1965.
- Y. Makino, R. Cao, K. Svensson et al., “Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression,” Nature, vol. 414, no. 6863, pp. 550–554, 2001.
- Y. Makino, R. Uenishi, K. Okamoto et al., “Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells,” Journal of Biological Chemistry, vol. 282, no. 19, pp. 14073–14082, 2007.
- D. W. Nebert, A. L. Roe, M. Z. Dieter, W. A. Solis, Y. Yang, and T. P. Dalton, “Role of the aromatic hydrocarbon receptor and [Ah] gene battery in the oxidative stress response, cell cycle control, and apoptosis,” Biochemical Pharmacology, vol. 59, no. 1, pp. 65–85, 2000.
- R. B. Hough and J. Piatigorsky, “Preferential transcription of rabbit Aldh1a1 in the cornea: implication of hypoxia-related pathways,” Molecular and Cellular Biology, vol. 24, no. 3, pp. 1324–1340, 2004.
- J. S. Boesch, R. Miskimins, W. K. Miskimins, and R. Lindahl, “The same xenobiotic response element is required for constitutive and inducible expression of the mammalian aldehyde dehydrogenase-3 gene,” Archives of Biochemistry and Biophysics, vol. 361, no. 2, pp. 223–230, 1999.
- M. C. Zhang, Y. Wang, and Y. Yang, “The expression of nuclear factor κ B in inflammation-induced rat corneal neovascularization,” Ocular Immunology and Inflammation, vol. 14, no. 6, pp. 359–365, 2006.
- G. Alexander, H. Carlsen, and R. Blomhoff, “Corneal NF-κB activity is necessary for the retention of transparency in the cornea of UV-B-exposed transgenic reporter mice,” Experimental Eye Research, vol. 82, no. 4, pp. 700–709, 2006.
- K. Kimura, S. Teranishi, K. Fukuda, K. Kawamoto, and T. Nishida, “Delayed disruption of barrier function in cultured human corneal epithelial cells induced by tumor necrosis factor-α in a manner dependent on NF-kB,” Investigative Ophthalmology & Visual Science, vol. 49, no. 2, pp. 565–571, 2008.
- G. Sosne, P. Qiu, P. L. Christopherson, and M. K. Wheater, “Thymosin beta 4 suppression of corneal NFκB: a potential anti-inflammatory pathway,” Experimental Eye Research, vol. 84, no. 4, pp. 663–669, 2007.
- G. Sosne, P. Qiu, P. L. Christopherson, and M. K. Wheater, “Thymosin beta 4 suppression of corneal NFκB: a potential anti-inflammatory pathway,” Experimental Eye Research, vol. 84, no. 4, pp. 663–669, 2007.
- M. L. Goodkin, S. Epstein, P. A. Asbell, and J. A. Blaho, “Nuclear translocation of NF-κB precedes apoptotic poly(ADP-ribose) polymerase cleavage during productive HSV-1 replication in corneal epithelial cells,” Investigative Ophthalmology & Visual Science, vol. 48, no. 11, pp. 4980–4988, 2007.
- V. Bitko, N. E. Garmon, T. Cao et al., “Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection,” BMC Microbiology, vol. 4, article 28, 2004.
- J. Rajaiya, N. Sadeghi, and J. Chodosh, “Specific NFkappaB subunit activation and kinetics of cytokine induction in adenoviral keratitis,” Molecular vision, vol. 15, pp. 2879–2889, 2009.
- T. Orita, K. Kimura, H. Y. Zhou, and T. Nishida, “Poly(I:C)-induced adhesion molecule expression mediated by (NF)-κband phosphoinositide 3-Kinase-Akt signaling pathways in human corneal fibroblasts,” Investigative Ophthalmology & Visual Science, vol. 51, no. 11, pp. 5556–5560, 2010.
- K. Yoshida, Y. Hu, and M. Karin, “IκB kinase α is essential for development of the mammalian cornea and conjunctiva,” Investigative Ophthalmology & Visual Science, vol. 41, no. 12, pp. 3665–3669, 2000.
- M. Ueta, J. Hamuro, M. Yamamoto, K. Kaseda, S. Akira, and S. Kinoshita, “Spontaneous ocular surface inflammation and goblet cell disappearance in IκBζ gene-disrupted mice,” Investigative Ophthalmology & Visual Science, vol. 46, no. 2, pp. 579–588, 2005.
- X. Lou, S. Sun, W. Chen et al., “Negative feedback regulation of NF-κB action by CITED2 in the nucleus,” Journal of Immunology, vol. 186, no. 1, pp. 539–548, 2011.
- Y. Chen, E. C. Carlson, Z. Y. Chen et al., “Conditional deletion of Cited2 results in defective corneal epithelial morphogenesis and maintenance,” Developmental Biology, vol. 334, no. 1, pp. 243–252, 2009.
- S. J. Freedman, Z. Y. J. Sun, A. L. Kung, D. S. France, G. Wagner, and M. J. Eck, “Structural basis for negative regulation of hypoxia-inducible factor-1α by CITED2,” Nature Structural Biology, vol. 10, no. 7, pp. 504–512, 2003.
- R. Ohlsson, R. Renkawitz, and V. Lobanenkov, “CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease,” Trends in Genetics, vol. 17, no. 9, pp. 520–527, 2001.
- T. H. Kim, Z. K. Abdullaev, A. D. Smith et al., “Analysis of the Vertebrate Insulator Protein CTCF-Binding Sites in the Human Genome,” Cell, vol. 128, no. 6, pp. 1231–1245, 2007.
- T. Li and L. Lu, “Epidermal growth factor-induced proliferation requires down-regulation of Pax6 in corneal epithelial cells,” Journal of Biological Chemistry, vol. 280, no. 13, pp. 12988–12995, 2005.
- T. Li, Z. Lu, and L. Lu, “Regulation of eye development by transcription control of CCCTC Binding Factor (CTCF),” Journal of Biological Chemistry, vol. 279, no. 26, pp. 27575–27583, 2004.
- D. Wu, T. Li, Z. Lu, W. Dai, M. Xu, and L. Lu, “Effect of CTCF-binding motif on regulation of PAX6 transcription,” Investigative Ophthalmology & Visual Science, vol. 47, pp. 2422–2429, 2006.
- T. Li and L. Lu, “Functional role of CCCTC binding factor (CTCF) in stress-induced apoptosis,” Experimental Cell Research, vol. 313, no. 14, pp. 3057–3065, 2007.
- Y. Wang and L. Lu, “Activation of oxidative stress-regulated Bcl-3 suppresses CTCF in corneal epithelial cells,” PLoS One, vol. 6, article e23984, 2011.
- L. Wang, S. X. Deng, and L. Lu, “Role of CTCF in EGF-induced migration of immortalized human corneal epithelial cells,” Investigative Ophthalmology & Visual Science, vol. 53, pp. 946–951, 2012.
- J. Wang, Y. Wang, and L. Lu, “De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human corneal epithelial cells,” The Journal of Biological Chemistry, vol. 287, pp. 12469–12479, 2012.
- N. Hahn, C. T. Dietz, S. Kuhl, U. Vossmerbaeumer, and J. Kroll, “KLEIP deficiency in mice causes progressive corneal neovascular dystrophy,” Investigative Ophthalmology & Visual Science, vol. 53, pp. 3260–3268, 2012.
- A. M. Verdoni, N. Aoyama, A. Ikeda, and S. Ikeda, “Effect of destrin mutations on the gene expression profile in vivo,” Physiological Genomics, vol. 34, no. 1, pp. 9–21, 2008.
- R. S. Smith, N. L. Hawes, S. D. Kuhlmann et al., “Corn1: a mouse model for corneal surface disease and neovascularization,” Investigative Ophthalmology & Visual Science, vol. 37, no. 2, pp. 397–404, 1996.
- I. J. Wang, C. W. C. Kao, C. Y. Liu et al., “Characterization of Corn1 Mice: alteration of epithelial and stromal cell gene expression,” Molecular Vision, vol. 7, pp. 20–26, 2001.
- S. Ikeda, L. A. Cunningham, D. Boggess et al., “Aberrant actin cytoskeleton leads to accelerated proliferation of corneal epithelial cells in mice deficient for destrin (actin depolymerizing factor),” Human Molecular Genetics, vol. 12, pp. 1029–1037, 2003.
- C. Cursiefen, S. Ikeda, P. M. Nishina et al., “Spontaneous corneal hem- and lymphangiogenesis in mice with destrin-mutation depend on VEGFR3 signaling,” American Journal of Pathology, vol. 166, no. 5, pp. 1367–1377, 2005.
- A. M. Verdoni, R. S. Smith, A. Ikeda, and S. Ikeda, “Defects in actin dynamics lead to an autoinflammatory condition through the upregulation of CXCL5,” PLoS ONE, vol. 3, no. 7, article e2701, Article ID e2701, 2008.
- A. M. Verdoni, K. J. Schuster, B. S. Cole, A. Ikeda, W. W. Kao, and S. Ikeda, “A pathogenic relationship between a regulator of the actin cytoskeleton and serum response factor,” Genetics, vol. 186, no. 1, pp. 147–157, 2010.
- S. K. Swamynathan, M. A. Crawford, W. G. Robison, J. Kanungo, and J. Piatigorsky, “Adaptive differences in the structure and macromolecular compositions of the air and water corneas of the "four-eyed" fish (Anableps anableps),” FASEB Journal, vol. 17, no. 14, pp. 1996–2005, 2003.
- S. Jia, M. Omelchenko, D. Garland et al., “Duplicated gelsolin family genes in zebrafish: a novel scinderin-like gene (scinla) encodes the major corneal crystallin,” FASEB Journal, vol. 21, no. 12, pp. 3318–3328, 2007.
- J. Kanungo, S. K. Swamynathan, and J. Piatigorsky, “Abundant corneal gelsolin in Zebrafish and the “four-eyed” fish, Anableps anableps: possible analogy with multifunctional lens crystallins,” Experimental Eye Research, vol. 79, no. 6, pp. 949–956, 2004.
- J. Kanungo, Z. Kozmik, S. K. Swamynathan, and J. Piatigorsky, “Gelsolin is a dorsalizing factor in zebrafish,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 6, pp. 3287–3292, 2003.
- J. P. Solomon, L. J. Page, W. E. Balch, and J. W. Kelly, “Gelsolin amyloidosis: genetics, biochemistry, pathology and possible strategies for therapeutic intervention,” Critical Reviews in Biochemistry and Molecular Biology, vol. 47, pp. 282–296, 2012.
- M. E. Rosenberg, T. M. T. Tervo, J. Gallar et al., “Corneal morphology and sensitivity in lattice dystrophy type II (familial amyloidosis, Finnish type),” Investigative Ophthalmology & Visual Science, vol. 42, no. 3, pp. 634–641, 2001.
- C. P. J. Maury, “Finnish amyloidosis: from protein to gene,” Journal of Internal Medicine, vol. 230, no. 3, pp. 193–194, 1991.
- C. P. J. Maury, “Gelsolin-related amyloidosis. Identification of the amyloid protein in Finnish hereditary amyloidosis as a fragment of variant gelsolin,” Journal of Clinical Investigation, vol. 87, no. 4, pp. 1195–1199, 1991.
- W. T. Kays and J. Piatigorsky, “Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 25, pp. 13594–13599, 1997.
- J. Davis, D. Davis, B. Norman, and J. Piatigorsky, “Gene expression of the mouse corneal crystallin Aldh3a1: activation by Pax6, Oct1, and p300,” Investigative Ophthalmology & Visual Science, vol. 49, no. 5, pp. 1814–1826, 2008.
- R. Manzer, L. Qamar, T. Estey, A. Pappa, D. R. Petersen, and V. Vasiliou, “Molecular cloning and baculovirus expression of the rabbit corneal aldehyde dehydrogenase (ALDH1A1) cDNA,” DNA and Cell Biology, vol. 22, no. 5, pp. 329–338, 2003.
- C. M. Sax, C. Salamon, W. T. Kays et al., “Transketolase is a major protein in the mouse cornea,” Journal of Biological Chemistry, vol. 271, no. 52, pp. 33568–33574, 1996.
- C. M. Sax, W. T. Kays, C. Salamon, M. M. Chervenak, Y. S. Xu, and J. Piatigorsky, “Transketolase gene expression in the cornea is influenced by environmental factors and developmentally controlled events,” Cornea, vol. 19, no. 6, pp. 833–841, 2000.
- W. W. Y. Kao, C. Y. Liu, R. L. Converse et al., “Keratin 12-deficient mice have fragile corneal epithelia,” Investigative Ophthalmology & Visual Science, vol. 37, no. 13, pp. 2572–2584, 1996.
- A. Shiraishi, R. L. Converse, C. Y. Liu, F. Zhou, C. W. C. Kao, and W. W. Y. Kao, “Identification of the cornea-specific keratin 12 promoter by in vivo particle-mediated gene transfer,” Investigative Ophthalmology & Visual Science, vol. 39, no. 13, pp. 2554–2561, 1998.
- J. J. Liu, W. W. Y. Kao, and S. E. Wilson, “Corneal epithelium-specific mouse keratin K12 promoter,” Experimental Eye Research, vol. 68, no. 3, pp. 295–301, 1999.
- S. Chakravarti, T. Magnuson, J. H. Lass, K. J. Jepsen, C. LaMantia, and H. Carroll, “Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican,” Journal of Cell Biology, vol. 141, no. 5, pp. 1277–1286, 1998.
- S. Ying, A. Shiraishi, C. W. C. Kao et al., “Characterization and expression of the mouse lumican gene,” Journal of Biological Chemistry, vol. 272, no. 48, pp. 30306–30313, 1997.
- S. Chakravarti, W. M. Petroll, J. R. Hassell et al., “Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma,” Investigative Ophthalmology & Visual Science, vol. 41, no. 11, pp. 3365–3373, 2000.
- S. Chakravarti, G. Zhang, I. Chervoneva, L. Roberts, and D. E. Birk, “Collagen fibril assembly during postnatal development and dysfunctional regulation in the lumican-deficient murine cornea,” Developmental Dynamics, vol. 235, no. 9, pp. 2493–2506, 2006.
- S. Lee, K. Bowrin, A. R. Hamad, and S. Chakravarti, “Extracellular matrix lumican deposited on the surface of neutrophils promotes migration by binding to β2 integrin,” Journal of Biological Chemistry, vol. 284, no. 35, pp. 23662–23669, 2009.
- K. Lohr, H. Sardana, S. Lee et al., “Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis,” Inflammatory Bowel Diseases, vol. 18, pp. 143–151, 2012.
- A. J. Quantock, K. M. Meek, and S. Chakravarti, “An x-ray diffraction investigation of corneal structure in lumican-deficient mice,” Investigative Ophthalmology & Visual Science, vol. 42, no. 8, pp. 1750–1756, 2001.
- H. Shao, S. Lee, S. Gae-Scott et al., “Extracellular matrix lumican promotes bacterial phagocytosis and Lum-/- mice show increased Pseudomonas aeruginosa lung infection severity,” The Journal of Biological Chemistry, vol. 287, no. 43, pp. 35860–35872, 2012.
- F. Wu, N. Vij, L. Roberts, S. Lopez-Briones, S. Joyce, and S. Chakravarti, “A novel role of the lumican core protein in bacterial lipopolysaccharide- induced innate immune response,” Journal of Biological Chemistry, vol. 282, no. 36, pp. 26409–26417, 2007.
- E. C. Carlson, C. Y. Liu, T. I. Chikama et al., “Keratocan, a cornea-specific keratan sulfate proteoglycan, is regulated by lumican,” Journal of Biological Chemistry, vol. 280, no. 27, pp. 25541–25547, 2005.
- C. Y. Liu, D. E. Birk, J. R. Hassell, B. Kane, and W. W. Y. Kao, “Keratocan-deficient mice display alterations in corneal structure,” Journal of Biological Chemistry, vol. 278, no. 24, pp. 21672–21677, 2003.
- C. Y. Liu, A. Shiraishi, C. W. C. Kao et al., “The cloning of mouse keratocan cDNA and genomic DNA and the characterization of its expression during eye development,” Journal of Biological Chemistry, vol. 273, no. 35, pp. 22584–22588, 1998.
- C. Y. Liu, H. Arar, C. Kao, and W. W. Y. Kao, “Identification of a 3.2 kb 5'-flanking region of the murine keratocan gene that directs β-galactosidase expression in the adult corneal stroma of transgenic mice,” Gene, vol. 250, no. 1-2, pp. 85–96, 2000.
- O. Dahan, H. Gingold, and Y. Pilpel, “Regulatory mechanisms and networks couple the different phases of gene expression,” Trends in Genetics, vol. 27, no. 8, pp. 316–322, 2011.
- A. R. Morris, N. Mukherjee, and J. D. Keene, “Systematic analysis of posttranscriptional gene expression,” Wiley Interdisciplinary Reviews, vol. 2, no. 2, pp. 162–180, 2010.
Copyright © 2013 Shivalingappa K. Swamynathan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.