Essential Role of Thioredoxin 2 in Mitigating Oxidative Stress in Retinal Epithelial Cells

Figure 5

Western blot analysis of heat shock proteins expressed by cells under oxidative stress. Cells were exposed to 4-HNE for 3 hours. Untreated cells served as controls. The same blots were reprobed with an anti-β-actin antibody to serve as loading controls. Hsp70 (*) or Hsp 90 (**) expression in ARPE-Trx2 cells was compared with that of untransfected or ARPE-pmCherry cells that were exposed to 4-HNE for 3 hours. Densitometric analysis of these proteins was performed using Quantity One software. The value relative to β-actin of untreated cells is defined as 1.