Research Article
Optimization of Storage Temperature for Cultured ARPE-19 Cells
Table 1
Characterization of retinal pigment epithelial cells.
| Markers | Specificity | Investigator criteria for positive staining | Investigator A and B agreement | Significance level of correlation (r) between investigators | 95% CI of difference between investigators |
| RPE65 | Differentiated cells (cytosol/membrane) | Stained cytosol | | −0.1% to +0.0% | PCNA | Proliferating cells (nucleus) | Stained nucleus | | −3.3% to +0.8% | Caspase-3 | Apoptotic cells (mainly cytosol) | Stained cytosol | | −0.2% to +0.0% |
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CI: confidence interval; r: Pearson’s correlation coefficient.
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