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Journal of Ophthalmology
Volume 2013 (2013), Article ID 309192, 4 pages
Clinical Study

Autofluorescence Images with Carl Zeiss versus Topcon Eye Fundus Camera: A Comparative Study

1Instituto de Oftalmobiologia Aplicada (IOBA), Universidad de Valladolid, Spain
2Consultorio 225, Consultorios América, Vía España, Panamá, Panama
3Complejo Asistencial de Palencia, Spain

Received 18 January 2013; Revised 1 April 2013; Accepted 2 April 2013

Academic Editor: Atsushi Hayashi

Copyright © 2013 Juan M. Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To compare the autofluorescence images of the Zeiss versus Topcon eye fundus cameras and design an objective way to quantify it. Procedures. The IMAGEJ software was used to determine the gray level corresponding to the darkest veins and the peripapillary ring (thresholds), the level of white of the brightest perifoveal area, their difference (contrast level), and the suprathreshold area for each photograph. Results. Carl Zeiss has higher contrast values than Topcon. The Topcon contrast presented a crest with further decline as the suprathreshold area continued to increase. On the contrary, the Zeiss profile did not decline in contrast. Conclusions and Message. The Carl Zeiss camera showed superior contrast ability over the Topcon when performing autofluorescence imaging. We set objective parameters to compare fundus cameras FAF images. These parameters could be the base to objectively measure and determine changes and realize followup to areas of hyper- or hypofluorescence.