RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β₂ Induced Proliferation in Human Tenon Capsule Fibroblasts

Table 1

Targets and duplex sequences for human CTGF specific siRNAs and control siRNA.

siRNA duplex	CTGF target sequence(5′-3′)	siRNA duplex sequences
CTGF-siRNA1	(1024)GCACCAGCATGAAGACATACC	5′-GCACCAGCAUGAAGACAUACCdTdT-3′
		5′-GGUAUGUCUUCAUGCUGGUGCdTdT-3′
CTGF-siRNA2	(862)CCCGGGTTACCAATGACAACG	5′-CCCGGGUUACCAAUGACAACGdTdT-3′
		5′-CGUUGUCAUUGGUAACCCGGGdTdT-3′
CTGF-siRNA3	(883)CCTCCTGCAGGCTAGAGAAGC	5′-CCUCCUGCAGGCUAGAGAAGCdTdT-3′
		5′-CCAAGCCUAUCAAGUUUGAGCdTdT-3′
CTGF-siRNA4	(994)CCAAGCCTATCAAGTTTGAGC	5′-CCAAGCCUAUCAAGUUUGAGCdTdT-3′
		5′-GCUCAAACUUGAUAGGCUUGGdTdT-3′
control siRNA		5′-UUCUCCGAACGUGUCACGUdTdT-3′
		5′-ACUCCUCGCAGCAUUUCCCGGdTdT-3′

Four siRNAs were designed from the coding sequence of human CTGF gene. The target sequences (5′-3′) and the siRNA duplex sequences are listed, with the position of the first nucleotide in CTGF sequence shown in parentheses. A nonspecific, scrambled siRNA duplex as control siRNA was used as a control.