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Journal of Ophthalmology
Volume 2014, Article ID 304694, 11 pages
Research Article

Novel Lutein Loaded Lipid Nanoparticles on Porcine Corneal Distribution

1Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33302, Taiwan
2Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, 261 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33303, Taiwan
3College of Medicine, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33302, Taiwan
4Department of Ophthalmology, Chang Gung Memorial Hospital, 5 Fusing Street, Kwei-Shan, Tao-Yuan 33305, Taiwan

Received 3 April 2014; Revised 6 June 2014; Accepted 10 June 2014; Published 2 July 2014

Academic Editor: Miltiadis Tsilimbaris

Copyright © 2014 Chi-Hsien Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Topical delivery has the advantages including being user friendly and cost effective. Development of topical delivery carriers for lutein is becoming an important issue for the ocular drug delivery. Quantification of the partition coefficient of drug in the ocular tissue is the first step for the evaluation of delivery efficacy. The objectives of this study were to evaluate the effects of lipid nanoparticles and cyclodextrin (CD) on the corneal lutein accumulation and to measure the partition coefficients in the porcine cornea. Lipid nanoparticles combined with 2% HPβCD could enhance lutein accumulation up to (μg/g) which is 4.9-fold higher than that of the nanoparticles. CD combined nanoparticles have 68% of drug loading efficiency and lower cytotoxicity in the bovine cornea cells. From the confocal images, this improvement is due to the increased partitioning of lutein to the corneal epithelium by CD in the lipid nanoparticles. The novel lipid nanoparticles could not only improve the stability and entrapment efficacy of lutein but also enhance the lutein accumulation and partition in the cornea. Additionally the corneal accumulation of lutein was further enhanced by increasing the lutein payload in the vehicles.