Journal of Ophthalmology / 2015 / Article / Fig 3

Research Article

Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis

Figure 3

The cellular localization of apelin/APJ. (a) Apelin immunoreactivity was found weak and diffused in the cytoplasm of normal (A) but showed more intense cytoplasmic staining in hypoxic pericyte (C). Scale bar = 100 μm. Similarly, (b) compared with restricted cytomembrane expression in normal (A) and hypoxia (C) pericyte, APJ localization expanded and brightened in the cytoplasm and cytoplasm membrane (apelin in red, APJ in green, and DAPI in blue). Scale bar = 100 μm. (c) and (d) Western blot analysis shows that the expression of apelin and APJ under hypoxia was upregulated 2.5-fold and 1.9-fold, respectively ().