Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
Morphology of the RSCs and Il-17rc expression. (a) RSCs are spindle-shaped even after passaging (scale bar: 200 μm). (b) Il-17rc mRNA was induced after the stimulation of IL-1β, IL18, or IL-17A in a dose-dependent manner. (c) IL-17rc protein (green) is weakly expressed in nonstimulated RSCs, but more highly expressed after stimulation with IL-1β (100 ng/mL), IL18 (10 ng/mL), or IL-17A (10 ng/mL). The nuclei were stained with DAPI (blue) (scale bar: 20 μm). (d) Il-17rc mRNA was induced after the stimulation of 100 ng/mL IL-1β, 10 ng/mL IL-18, or 10 ng/mL IL-17A in primary cultured mouse PRE cells. compared to control. compared to control.