Figure 4: Relative methylation status and expression of protein levels of WRN in HLEB-3 after treatment with 5-aza-dC. In the following panels, each row of circles represents a single clone. Open and close circles represent unmethylated and methylated CpG sites, respectively. (a) After treatment with 5-aza-dC, the methylation rate of WRN in HLEB-3 was decreased. (b) Protein expression of WRN in the untreated control cells and in cells after treatment with 5-aza-dC. Relative WRN protein level to GAPDH is presented as mean ± SD. .