Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea

Figure 1

Tube-like structure formation by human umbilical vein endothelial cells (HUVECs). (a) The HUVEC culture on fibroblast feeder was processed for CD31 immunocytochemistry at day 11. In control vascular endothelial growth factor- (VEGF-) plus, culture HUVEC forms CD31-labeled tube-like tissue (A). VEGF action of tube-like structure formation is not affected by supplementation of a TRPV1 antagonist, SB366791 (10 μM) (B). Measurement of total length (b) and the number of branching points (c) of CD31-labeled structure coincide with the findings shown in frame (a). < 0.05, bar, 1 mm.

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