Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea

Figure 4

Immunohistochemical detection of angiogenic or inflammatory components in centrally cauterized cornea. Active form of TGFβ1 was detected in stroma just beneath the epithelium in the area of cauterization at days 3 and 7 in WT mice (arrows in (a) and (c)). Its immunoreactivity was quite less marked in cornea of KO mice ((b) and (d)). At day 1 after cauterization very faint VEGF immunoreactivity was observed in basal cells of corneal epithelia in cauterization area of both WT (e) and KO (f) mice. At day 3 the basal epithelial cell with VEGF immunoreactivity was more frequently observed in a WT cornea (e) as compared with a KO mouse (f). Immunoreactivity for substance P was detected in basal layer of corneal epithelium with no obvious difference in intensity between WT and KO mice at days 1 and 3 ((g)–(l)). Bar, 20 μm.

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