(a)
(b)
(c)
(d)
Figure 8: E-cadherin role in CAOMECS epithelial barrier. (a) Picture of CAOMECS commencing to detach when treated with 2.5 mM EGTA (white arrow) and completely lost adhesion when treated with 5 mM EGTA (black arrow). (b) is a semiquantitative measurement of E-cadherin. Note that E-cadherin levels were significantly reduced when CAOMECS grafts were treated with EGTA. (c) is an illustration of Cascade Blue experiment design where the probe was only added in cell culture media of CAOMECS. The graph in (d) showed the levels of Cascade Blue in media and in CAOMECS cells compared to controls (CAOMECS culture without Cascade Blue addition). Note that fluorescence did not traverse the barrier of CAOMECS.