Research Article
Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation
Table 1
Methylation primer sequences and annealing temperature.
| | Genes | Sequences (5′-3′) | Annealing temperature (°C) | Product size |
| | LATS1 M | F: GGAGTTT CGTTTTGTC | 53°C | 138 bp | | R: CGACGTAATAACG AACGCCTA |
| | LATS1 U | F: TAGGTTGGAGTGTGGTGGT | 57°C | 121 bp | | R: CCC AACATAATAACAAACACCT |
| | LATS2 M | F: ATTTCGGTTTATTGTAATTTTC | 55°C | 148 bp | | R: AACCAACATAATAAAACCCCG |
| | LATS2 U | F: TTTGTTTTTTGGGTTTAAGT | 55°C | 130 bp | | R: CCAACATAATA AAACCCCA |
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M, methylated; U, unmethylated.
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