Research Article

SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes

Figure 4

High glucose induced DBC1 sumoylation in BRPCs. (a) After HEK293T cells were transfected with Flag-DBC1, with or without RH-SUMO3 as indicated for 48 h, cell lysates were subjected to immunoprecipitation with anti-Flag antibody followed by immunoblot analysis. (b) BRPCs were treated with normal (NG) or high glucose (HG) for 48 h. Their lysates were subjected to immunoprecipitation with IgG or anti-DBC1 antibody followed by immunoblot analysis.
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