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Figure 1: The culture of human limbal epithelial cells in SR medium. (a) The human corneal epithelial cells formed tightly compacted colonies in FAD medium after seeding cells for 3 days. (b) After 7 days, epithelial cells reached confluence in FAD medium with uniform small size. (c) Human corneal epithelial cells formed colonies within SR medium, which were similar to those formed within FAD medium, but these colonies were less tightly compacted than those in FAD. (d) After 7 days, epithelial cells reached confluence in SR medium with uniform small size which was similar to those in FAD culture Original magnification: (a) ×10; (b) ×10 (scale bars: 100 μm).